Detailed information of TA system TA089602

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 221983..222198
• Replicon: chromosome
• Accession: NZ_CP025046
• Organism: Clostridioides difficile strain W0022a

Toxin (Protein)

• Gene name: CD2889
• Uniprot ID: Q183X5
• Locus tag: CWR55_RS01335
• Protein ID: WP_011861731.1
• Coordinates: 221983..222126 (+)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	RCd11
Locus tag	-
Coordinates	222048..222198 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWR55_RS01305	218499..218729	+	231	WP_021364192.1	hemolysin XhlA family protein	-
CWR55_RS01310	218749..219006	+	258	WP_009898413.1	phage holin family protein	-
CWR55_RS01315	219006..219818	+	813	WP_016056343.1	N-acetylmuramoyl-L-alanine amidase	-
CWR55_RS01320	220068..220559	+	492	WP_016056344.1	hypothetical protein	-
CWR55_RS01325	220561..221127	+	567	WP_016056345.1	hypothetical protein	-
CWR55_RS01330	221537..221725	-	189	WP_009898401.1	hypothetical protein	-
CWR55_RS01335	221983..222126	+	144	WP_011861731.1	hypothetical protein	Toxin
-	222048..222198	-	151	NuclAT_1	-	Antitoxin
-	222052..222198	-	147	NuclAT_2	-	-
CWR55_RS01340	222634..223023	+	390	WP_021376798.1	BlaI/MecI/CopY family transcriptional regulator	-
CWR55_RS01345	223117..223500	+	384	WP_021364164.1	BlaI/MecI/CopY family transcriptional regulator	-
CWR55_RS01355	224179..225609	+	1431	WP_021362134.1	metallophosphoesterase	-
CWR55_RS01360	225757..226107	-	351	Protein_221	LysR family transcriptional regulator	-
CWR55_RS01365	226102..226623	+	522	WP_011860666.1	chromate transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	groEL	162357..223500	61143

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-134)

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5399.34 Da
• Isoelectric Point: 10.8277

>T89602 WP_011861731.1 NZ_CP025046:221983-222126 [Clostridioides difficile]
MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNKKRH

Nucleotide

• Length: 144 bp

>T89602 NZ_CP025046:221983-222126 [Clostridioides difficile]
ATGAGCGAATTTTTACTAGGAGTGTTAGCTAGTTTAACAGCTAGCTTTATTACATATATTATTTCCAGAAAAGTAAAAAG
CCACTCTGGCAGGAGTGACTTTGAGCTTGATGTAAAAATCAAGTTTAATAAAAAACGACATTAA

Antitoxin

Nucleotide

• Length: 151 bp

>AT89602 NZ_CP025046:c222198-222048 [Clostridioides difficile]
GTAAATTCGTATCAAAAACAAAAAAAGAAACTACAATCTATTAGCCTAGAGTGAAGTTTCATTAACTAAATATTAATGTC
GTTTTTTATTAAACTTGATTTTTACATCAAGCTCAAAGTCACTCCTGCCAGAGTGGCTTTTTACTTTTCTG

Structures

Toxin

• AlphaFold DB: A0A069A3R1

Antitoxin