Detailed information of TA system TA089596

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2435158..2435360
• Replicon: chromosome
• Accession: NZ_CP025045
• Organism: Clostridioides difficile strain W0023a

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: CWR56_RS11415
• Protein ID: WP_004454589.1
• Coordinates: 2435208..2435360 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	2435158..2435287 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWR56_RS11385	2430462..2430926	+	465	WP_004454576.1	hypothetical protein	-
CWR56_RS11395	2431255..2431416	-	162	WP_004454578.1	hypothetical protein	-
CWR56_RS19105	2431468..2432281	-	814	Protein_2156	toxin Bro	-
CWR56_RS19110	2432402..2432554	-	153	WP_009897477.1	hypothetical protein	-
CWR56_RS11410	2434091..2435014	-	924	WP_004454587.1	SHOCT domain-containing protein	-
-	2435158..2435287	+	130	NuclAT_1	-	Antitoxin
CWR56_RS11415	2435208..2435360	-	153	WP_004454589.1	hypothetical protein	Toxin
CWR56_RS11420	2435634..2435957	-	324	WP_009897482.1	hypothetical protein	-
CWR56_RS11425	2435993..2436247	-	255	WP_004454592.1	hypothetical protein	-
CWR56_RS11435	2436679..2437197	-	519	Protein_2162	transposase	-
CWR56_RS11440	2437487..2437861	-	375	Protein_2163	BlaI/MecI/CopY family transcriptional regulator	-
CWR56_RS11445	2437966..2438340	-	375	WP_004454597.1	BlaI/MecI/CopY family transcriptional regulator	-
CWR56_RS11450	2439146..2439634	+	489	WP_004454599.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
CWR56_RS19050	2440023..2440193	+	171	WP_004454601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2426885..2445443	18558

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T89596 WP_004454589.1 NZ_CP025045:c2435360-2435208 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T89596 NZ_CP025045:c2435360-2435208 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT89596 NZ_CP025045:2435158-2435287 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin