89215

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4086668..4087305 Replicon chromosome
Accession NZ_CP024901
Organism Photorhabdus laumondii subsp. laumondii strain TT01

Toxin (Protein)


Gene name hicA Uniprot ID Q7N1I2
Locus tag PluTT01m_RS17995 Protein ID WP_011147672.1
Coordinates 4087132..4087305 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1B8YHV7
Locus tag PluTT01m_RS17990 Protein ID WP_036808644.1
Coordinates 4086668..4087078 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PluTT01m_RS17955 4082267..4082764 + 498 WP_011147663.1 siphovirus Gp157 family protein -
PluTT01m_RS17960 4082816..4083004 + 189 WP_011147664.1 hypothetical protein -
PluTT01m_RS27160 4083574..4083744 + 171 WP_011147665.1 hypothetical protein -
PluTT01m_RS17965 4083749..4083964 + 216 WP_011147666.1 hypothetical protein -
PluTT01m_RS17970 4083916..4085099 + 1184 Protein_3429 DUF3596 domain-containing protein -
PluTT01m_RS26980 4085545..4085760 - 216 WP_049789790.1 hypothetical protein -
PluTT01m_RS17980 4085824..4086054 + 231 WP_011147669.1 hypothetical protein -
PluTT01m_RS17985 4086051..4086320 + 270 WP_011147670.1 helix-turn-helix domain-containing protein -
PluTT01m_RS17990 4086668..4087078 - 411 WP_036808644.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
PluTT01m_RS17995 4087132..4087305 - 174 WP_011147672.1 type II toxin-antitoxin system HicA family toxin Toxin
PluTT01m_RS18000 4087425..4089073 - 1649 Protein_3435 hypothetical protein -
PluTT01m_RS18005 4089073..4089609 - 537 WP_041380260.1 hypothetical protein -
PluTT01m_RS18010 4089581..4090189 - 609 WP_011147676.1 hypothetical protein -
PluTT01m_RS18015 4090309..4090928 - 620 Protein_3438 tail fiber assembly protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4007641..4096345 88704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-65)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6680.79 Da        Isoelectric Point: 11.3427

>T89215 WP_011147672.1 NZ_CP024901:c4087305-4087132 [Photorhabdus laumondii subsp. laumondii]
VKQSEFRRWLEAQGVEVSNGTNHLKLRYQGKRSIMPRHPSQEIKEPLRKAIIKQLGL

Download         Length: 174 bp

>T89215 NZ_CP024901:c4087305-4087132 [Photorhabdus laumondii subsp. laumondii]
GTGAAGCAAAGCGAGTTTAGACGGTGGCTGGAAGCTCAAGGCGTCGAAGTTTCAAATGGGACAAACCATCTGAAACTGAG
ATACCAGGGGAAACGAAGCATTATGCCGAGACACCCTAGCCAAGAGATCAAAGAGCCGCTGAGGAAAGCAATCATTAAGC
AATTAGGATTGTGA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15131.32 Da        Isoelectric Point: 4.2783

>AT89215 WP_036808644.1 NZ_CP024901:c4087078-4086668 [Photorhabdus laumondii subsp. laumondii]
MRYPVTLEPVEEGGYFVSFPDIPEALTQGDTREEALEMALDALITAFEFYFEDNEKIPLPGPVGQDYVDVPLSVASKVLM
LNAFLDSKLTQIELANRMGVKKQEVTRIFDLRHSTKIDTVGKVASAIGHQLTLSIE

Download         Length: 411 bp

>AT89215 NZ_CP024901:c4087078-4086668 [Photorhabdus laumondii subsp. laumondii]
ATGCGATATCCAGTCACACTAGAGCCAGTAGAAGAGGGCGGTTATTTCGTCTCATTCCCGGATATCCCCGAAGCTTTAAC
CCAGGGGGATACACGGGAAGAGGCGCTAGAGATGGCACTCGATGCGCTAATAACCGCTTTTGAATTTTACTTCGAAGATA
ATGAAAAAATCCCGCTTCCTGGCCCCGTAGGGCAGGATTATGTTGATGTGCCCTTAAGCGTGGCCTCTAAGGTGCTTATG
CTCAATGCGTTTCTTGATTCCAAGTTAACGCAAATTGAATTAGCCAACCGTATGGGGGTGAAAAAGCAGGAAGTGACCCG
TATCTTTGATTTACGGCACTCAACTAAAATTGATACGGTGGGTAAGGTCGCATCGGCTATCGGACATCAGTTAACTTTGT
CCATTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6L9JNW7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B8YHV7

References