Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3623547..3624226 Replicon chromosome
Accession NZ_CP024090
Organism Escherichia coli strain WG5

Toxin (Protein)


Gene name ykfI Uniprot ID S1FHS4
Locus tag CSR56_RS18495 Protein ID WP_000854680.1
Coordinates 3623885..3624226 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID S1EWR7
Locus tag CSR56_RS18490 Protein ID WP_000070396.1
Coordinates 3623547..3623864 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSR56_RS18445 3618985..3619806 + 822 WP_000197388.1 DUF945 domain-containing protein -
CSR56_RS18450 3620023..3620724 + 702 WP_000189411.1 WYL domain-containing protein -
CSR56_RS18455 3620765..3621001 + 237 WP_001144031.1 hypothetical protein -
CSR56_RS18460 3621001..3621444 + 444 WP_000649865.1 hypothetical protein -
CSR56_RS18465 3621467..3621934 + 468 WP_001385283.1 hypothetical protein -
CSR56_RS18470 3622011..3622250 + 240 WP_000194654.1 DUF905 domain-containing protein -
CSR56_RS18475 3622348..3622806 + 459 WP_000211838.1 antirestriction protein -
CSR56_RS18480 3622822..3623298 + 477 WP_000811693.1 RadC family protein -
CSR56_RS18485 3623307..3623528 + 222 WP_000691994.1 DUF987 domain-containing protein -
CSR56_RS18490 3623547..3623864 + 318 WP_000070396.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
CSR56_RS18495 3623885..3624226 + 342 WP_000854680.1 type IV toxin-antitoxin system toxin YkfI Toxin
CSR56_RS18505 3624798..3626051 - 1254 WP_000893272.1 glutamate-5-semialdehyde dehydrogenase -
CSR56_RS18510 3626063..3627166 - 1104 WP_001285288.1 glutamate 5-kinase -
CSR56_RS18515 3627454..3628509 + 1056 WP_045145434.1 phosphoporin PhoE -
CSR56_RS18520 3628548..3628949 - 402 WP_047402959.1 sigma factor-binding protein Crl -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3601416..3624446 23030


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12892.95 Da        Isoelectric Point: 9.6543

>T86708 WP_000854680.1 NZ_CP024090:3623885-3624226 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T86708 NZ_CP024090:3623885-3624226 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11982.62 Da        Isoelectric Point: 7.0270

>AT86708 WP_000070396.1 NZ_CP024090:3623547-3623864 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT86708 NZ_CP024090:3623547-3623864 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHS4

Antitoxin

Source ID Structure
AlphaFold DB S1EWR7

References