Detailed information of TA system TA075076

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3643882..3644561
• Replicon: chromosome
• Accession: NZ_CP020543
• Organism: Escherichia coli C

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: B6N50_RS18665
• Protein ID: WP_000854680.1
• Coordinates: 3644220..3644561 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: B6N50_RS18660
• Protein ID: WP_000070396.1
• Coordinates: 3643882..3644199 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6N50_RS18615 (B6N50_18615)	3639320..3640141	+	822	WP_000197388.1	DUF945 domain-containing protein	-
B6N50_RS18620 (B6N50_18620)	3640358..3641059	+	702	WP_000189411.1	WYL domain-containing protein	-
B6N50_RS18625 (B6N50_18625)	3641100..3641336	+	237	WP_001144031.1	hypothetical protein	-
B6N50_RS18630 (B6N50_18630)	3641336..3641779	+	444	WP_000649865.1	hypothetical protein	-
B6N50_RS18635 (B6N50_18635)	3641802..3642269	+	468	WP_001385283.1	hypothetical protein	-
B6N50_RS18640 (B6N50_18640)	3642346..3642585	+	240	WP_000194654.1	DUF905 domain-containing protein	-
B6N50_RS18645 (B6N50_18645)	3642683..3643141	+	459	WP_000211838.1	antirestriction protein	-
B6N50_RS18650 (B6N50_18650)	3643157..3643633	+	477	WP_000811693.1	RadC family protein	-
B6N50_RS18655 (B6N50_18655)	3643642..3643863	+	222	WP_000691994.1	DUF987 domain-containing protein	-
B6N50_RS18660 (B6N50_18660)	3643882..3644199	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
B6N50_RS18665 (B6N50_18665)	3644220..3644561	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
B6N50_RS18675 (B6N50_18675)	3645133..3646386	-	1254	WP_000893272.1	glutamate-5-semialdehyde dehydrogenase	-
B6N50_RS18680 (B6N50_18680)	3646398..3647501	-	1104	WP_001285288.1	glutamate 5-kinase	-
B6N50_RS18685 (B6N50_18685)	3647789..3648844	+	1056	WP_045145434.1	phosphoporin PhoE	-
B6N50_RS18690 (B6N50_18690)	3648883..3649284	-	402	WP_047402959.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP022154 Escherichia coli strain ABWA45 Genomic island TA078589 4235231..4262050 0.005 View NZ_CP024090 Escherichia coli strain WG5 Genomic island TA086708 3601416..3624446 0 View NZ_CP072565 Escherichia coli strain WG5::PA8 Genomic island TA198091 3665353..3688383 0 View NZ_CP072566 Escherichia coli strain WG5::TT12A Genomic island TA198114 3665703..3688058 0.001 View NZ_LT906474 Escherichia coli strain NCTC122 Genomic island TA293758 3620826..3643856 0	inside	Genomic island	-	-	3621751..3644781	23030

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T75076 WP_000854680.1 NZ_CP020543:3644220-3644561 [Escherichia coli C]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T75076 NZ_CP020543:3644220-3644561 [Escherichia coli C]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT75076 WP_000070396.1 NZ_CP020543:3643882-3644199 [Escherichia coli C]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT75076 NZ_CP020543:3643882-3644199 [Escherichia coli C]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7