Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/RHH-PIN |
| Location | 716410..717059 | Replicon | chromosome |
| Accession | NC_000962 | ||
| Organism | Mycobacterium tuberculosis H37Rv | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | P9WF77 |
| Locus tag | Rv0624 | Protein ID | NP_215138.1 |
| Coordinates | 716664..717059 (+) | Length | 132 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | P9WJ35 |
| Locus tag | Rv0623 | Protein ID | NP_215137.1 |
| Coordinates | 716410..716664 (+) | Length | 85 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Rv0620 | 712716..713807 | + | 1092 | NP_215134.1 | galactokinase | - |
| Rv0621 | 714202..715266 | + | 1065 | NP_215135.1 | membrane protein | - |
| Rv0622 | 715370..716317 | + | 948 | NP_215136.2 | membrane protein | - |
| Rv0623 | 716410..716664 | + | 255 | NP_215137.1 | antitoxin VapB30 | Antitoxin |
| Rv0624 | 716664..717059 | + | 396 | NP_215138.1 | ribonuclease VapC30 | Toxin |
| Rv0625c | 717153..717893 | - | 741 | NP_215139.1 | transmembrane protein | - |
| Rv0626 | 718025..718285 | + | 261 | NP_215140.1 | antitoxin VapB5 | - |
| Rv0627 | 718282..718689 | + | 408 | NP_215141.1 | ribonuclease VapC5 | - |
| Rv0628c | 718761..719912 | - | 1152 | NP_215142.1 | hypothetical protein | - |
| Rv0629c | 720005..721732 | - | 1728 | NP_215143.1 | exonuclease V subunit alpha RecD | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 132 a.a. Molecular weight: 14250.04 Da Isoelectric Point: 4.7475
>T81 NP_215138.1 NC_000962:716664-717059 [Mycobacterium tuberculosis H37Rv]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP
Download Length: 396 bp
>T81 NC_000962:716664-717059 [Mycobacterium tuberculosis H37Rv]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA
Antitoxin
Download Length: 85 a.a. Molecular weight: 9139.52 Da Isoelectric Point: 9.2702
>AT81 NP_215137.1 NC_000962:716410-716664 [Mycobacterium tuberculosis H37Rv]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA
Download Length: 255 bp
>AT81 NC_000962:716410-716664 [Mycobacterium tuberculosis H37Rv]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T3165 | Mycobacterium smegmatis str. MC2 155 |
81.25 |
99.225 |
0.806 |
| T79 | Mycobacterium tuberculosis H37Rv |
42.857 |
96.183 |
0.412 |
| T6246 | Mycobacterium tuberculosis H37Rv |
38.095 |
96.183 |
0.366 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT3165 | Mycobacterium smegmatis str. MC2 155 |
83.333 |
100 |
0.833 |
| AT79 | Mycobacterium tuberculosis H37Rv |
43.023 |
100 |
0.457 |
| AT6246 | Mycobacterium tuberculosis H37Rv |
40.698 |
100 |
0.417 |
Multiple sequence alignment
References
(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113]