Detailed information of TA system TA000079

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/RHH-PIN
• Location: 703244..703887
• Replicon: chromosome
• Accession: NC_000962
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF81
• Locus tag: Rv0609
• Protein ID: NP_215123.1
• Coordinates: 703486..703887 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ39
• Locus tag: Rv0608
• Protein ID: NP_215122.1
• Coordinates: 703244..703489 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Rv0601c	698524..698994	-	471	NP_215115.1	two component sensor kinase HK2	-
Rv0602c	699038..699799	-	762	NP_215116.1	two component DNA binding transcriptional regulator TcrA	-
Rv0603	699856..700167	+	312	NP_215117.1	hypothetical protein	-
Rv0604	700239..701189	+	951	NP_215118.1	lipoprotein LpqO	-
Rv0605	701430..702014	+	585	NP_215119.2	IS1536 family serine type transposase	-
Rv0607	702813..703199	+	387	NP_215121.1	hypothetical protein	-
Rv0608	703244..703489	+	246	NP_215122.1	antitoxin VapB28	Antitoxin
Rv0609	703486..703887	+	402	NP_215123.1	ribonuclease VapC28	Toxin
Rv0609A	703830..704057	+	228	YP_177628.1	hypothetical protein	-
Rv0610c	704752..705909	-	1158	NP_215124.1	hypothetical protein	-
Rv0611c	705961..706182	-	222	NP_215125.2	hypothetical protein	-
Rv0612	706324..706929	+	606	NP_215126.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T79 NP_215123.1 NC_000962:703486-703887 [Mycobacterium tuberculosis H37Rv]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T79 NC_000962:703486-703887 [Mycobacterium tuberculosis H37Rv]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT79 NP_215122.1 NC_000962:703244-703489 [Mycobacterium tuberculosis H37Rv]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT79 NC_000962:703244-703489 [Mycobacterium tuberculosis H37Rv]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T3165	Mycobacterium smegmatis str. MC2 155	46.032	97.674	0.45
T81	Mycobacterium tuberculosis H37Rv	42.857	96.183	0.412
T6246	Mycobacterium tuberculosis H37Rv	40	93.985	0.376

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT81	Mycobacterium tuberculosis H37Rv	43.023	100	0.457
AT3165	Mycobacterium smegmatis str. MC2 155	37.647	100	0.395

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: P9WF81

Antitoxin

• AlphaFold DB: P9WJ39

References

(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113]


 experimental literature