Detailed information of TA system TA076666

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 3253385..3253599
• Replicon: chromosome
• Accession: NZ_CP021447
• Organism: Clostridioides difficile strain DSM 104451

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: CDIF104451_RS15125
• Protein ID: WP_003429855.1
• Coordinates: 3253438..3253599 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	3253385..3253518 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF104451_RS15105 (3249503)	3249503..3250063	-	561	WP_032512002.1	ribosomal protein L7/L12	-
CDIF104451_RS15110 (3250495)	3250495..3250671	-	177	WP_165483698.1	hypothetical protein	-
CDIF104451_RS15115 (3250734)	3250734..3251561	-	828	WP_070114900.1	toxin Bro	-
CDIF104451_RS15120 (3251685)	3251685..3251852	-	168	WP_021370599.1	ribbon-helix-helix, copG family protein	-
- (3252935)	3252935..3253040	+	106	NuclAT_5	-	-
- (3252935)	3252935..3253040	+	106	NuclAT_5	-	-
- (3252935)	3252935..3253040	+	106	NuclAT_8	-	-
- (3252935)	3252935..3253040	+	106	NuclAT_9	-	-
- (3253385)	3253385..3253518	+	134	NuclAT_3	-	Antitoxin
- (3253385)	3253385..3253518	+	134	NuclAT_3	-	Antitoxin
- (3253385)	3253385..3253518	+	134	NuclAT_5	-	Antitoxin
- (3253385)	3253385..3253518	+	134	NuclAT_6	-	Antitoxin
CDIF104451_RS15125 (3253438)	3253438..3253599	-	162	WP_003429855.1	hypothetical protein	Toxin
CDIF104451_RS15130 (3253940)	3253940..3254380	-	441	WP_003429853.1	phage portal protein	-
CDIF104451_RS15135 (3254452)	3254452..3254922	-	471	WP_003429851.1	phage tail tube protein	-
CDIF104451_RS15140 (3254939)	3254939..3256249	-	1311	WP_021368245.1	phage tail sheath family protein	-
CDIF104451_RS15145 (3256251)	3256251..3256427	-	177	WP_021362376.1	hypothetical protein	-
CDIF104451_RS15150 (3256420)	3256420..3256857	-	438	WP_021362375.1	hypothetical protein	-
CDIF104451_RS15155 (3257206)	3257206..3258012	-	807	WP_021358994.1	hypothetical protein	-
CDIF104451_RS15160 (3258122)	3258122..3258412	-	291	WP_021358995.1	HK97 gp10 family phage protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T76666 WP_003429855.1 NZ_CP021447:c3253599-3253438 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T76666 NZ_CP021447:c3253599-3253438 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCATAAGTTCAAAT
AG

Antitoxin

Nucleotide

• Length: 134 bp

>AT76666 NZ_CP021447:3253385-3253518 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin