Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) CD-RCd/-
Location 2490043..2490245 Replicon chromosome
Accession NZ_CP021447
Organism Clostridioides difficile strain DSM 104451

Toxin (Protein)


Gene name CD2299.1 Uniprot ID Q185H1
Locus tag CDIF104451_RS11795 Protein ID WP_004454589.1
Coordinates 2490093..2490245 (-) Length 51 a.a.

Antitoxin (RNA)


Gene name SQ1641
Locus tag -
Coordinates 2490043..2490172 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDIF104451_RS11760 (2485425) 2485425..2485742 + 318 WP_009897465.1 hypothetical protein -
CDIF104451_RS11765 (2485878) 2485878..2486342 + 465 WP_011861514.1 hypothetical protein -
CDIF104451_RS11770 (2486670) 2486670..2486831 - 162 WP_004454578.1 hypothetical protein -
CDIF104451_RS11775 (2486883) 2486883..2487335 - 453 WP_009897470.1 hypothetical protein -
CDIF104451_RS11780 (2487332) 2487332..2487697 - 366 WP_009897471.1 Bro-N domain-containing protein -
CDIF104451_RS11785 (2487817) 2487817..2487969 - 153 WP_009897477.1 hypothetical protein -
CDIF104451_RS11790 (2489330) 2489330..2489824 - 495 WP_231310087.1 hypothetical protein -
- (2490043) 2490043..2490172 + 130 NuclAT_4 - Antitoxin
- (2490043) 2490043..2490172 + 130 NuclAT_4 - Antitoxin
- (2490043) 2490043..2490172 + 130 NuclAT_7 - Antitoxin
CDIF104451_RS11795 (2490093) 2490093..2490245 - 153 WP_004454589.1 hypothetical protein Toxin
CDIF104451_RS11800 (2490519) 2490519..2490842 - 324 WP_009897482.1 DUF6054 family protein -
CDIF104451_RS11805 (2490878) 2490878..2491132 - 255 WP_004454592.1 hypothetical protein -
CDIF104451_RS11810 (2491564) 2491564..2492082 - 519 Protein_2248 transposase -
CDIF104451_RS11815 (2492372) 2492372..2492746 - 375 Protein_2249 BlaI/MecI/CopY family transcriptional regulator -
CDIF104451_RS11820 (2492851) 2492851..2493225 - 375 WP_004454597.1 BlaI/MecI/CopY family transcriptional regulator -
CDIF104451_RS11825 (2494029) 2494029..2494517 + 489 WP_004454599.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
CDIF104451_RS11830 (2494902) 2494902..2495076 + 175 Protein_2252 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5817.92 Da        Isoelectric Point: 10.6365

>T76657 WP_004454589.1 NZ_CP021447:c2490245-2490093 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Download         Length: 153 bp

>T76657 NZ_CP021447:c2490245-2490093 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin


Download         Length: 130 bp

>AT76657 NZ_CP021447:2490043-2490172 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A125V725

Antitoxin

Download structure file

References