Detailed information of TA system TA076651

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1191945..1192159
• Replicon: chromosome
• Accession: NZ_CP021447
• Organism: Clostridioides difficile strain DSM 104451

Toxin (Protein)

• Gene name: CD2889
• Uniprot ID: Q183X5
• Locus tag: CDIF104451_RS05785
• Protein ID: WP_011861731.1
• Coordinates: 1191945..1192088 (+)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1192010..1192159 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF104451_RS05745 (1187403)	1187403..1187585	+	183	WP_021423106.1	hypothetical protein	-
CDIF104451_RS05750 (1187621)	1187621..1187851	+	231	WP_021423107.1	hemolysin XhlA family protein	-
CDIF104451_RS05755 (1187871)	1187871..1188128	+	258	WP_070114895.1	phage holin family protein	-
CDIF104451_RS05760 (1188128)	1188128..1188967	+	840	WP_070114896.1	N-acetylmuramoyl-L-alanine amidase	-
CDIF104451_RS05765 (1189532)	1189532..1190095	+	564	WP_021371073.1	hypothetical protein	-
CDIF104451_RS05770 (1190113)	1190113..1190544	+	432	WP_011861052.1	protein-export chaperone SecB	-
CDIF104451_RS05775 (1190546)	1190546..1191250	+	705	WP_070114897.1	hypothetical protein	-
CDIF104451_RS05780 (1191499)	1191499..1191687	-	189	WP_021360368.1	hypothetical protein	-
CDIF104451_RS05785 (1191945)	1191945..1192088	+	144	WP_011861731.1	hypothetical protein	Toxin
- (1192010)	1192010..1192159	-	150	NuclAT_3	-	Antitoxin
- (1192014)	1192014..1192159	-	146	NuclAT_1	-	-
- (1192014)	1192014..1192159	-	146	NuclAT_1	-	-
- (1192014)	1192014..1192159	-	146	NuclAT_1	-	-
CDIF104451_RS05790 (1192594)	1192594..1192971	+	378	WP_070114845.1	BlaI/MecI/CopY family transcriptional regulator	-
CDIF104451_RS05795 (1193081)	1193081..1193464	+	384	WP_015984867.1	BlaI/MecI/CopY family transcriptional regulator	-
CDIF104451_RS05800 (1193658)	1193658..1194233	+	576	WP_021360401.1	GntR family transcriptional regulator	-
CDIF104451_RS05805 (1194259)	1194259..1194672	+	414	WP_009896035.1	PTS sugar transporter subunit IIA	-
CDIF104451_RS05810 (1194676)	1194676..1195419	+	744	WP_003437997.1	BtpA/SgcQ family protein	-
CDIF104451_RS05815 (1195419)	1195419..1195889	+	471	WP_003428544.1	PTS sugar transporter subunit IIB	-
CDIF104451_RS05820 (1195906)	1195906..1196664	+	759	WP_009888882.1	PTS sugar transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5399.34 Da
• Isoelectric Point: 10.8277

>T76651 WP_011861731.1 NZ_CP021447:1191945-1192088 [Clostridioides difficile]
MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNKKRH

Nucleotide

• Length: 144 bp

>T76651 NZ_CP021447:1191945-1192088 [Clostridioides difficile]
ATGAGCGAATTTTTACTAGGAGTGTTAGCTAGTTTAACAGCTAGCTTTATTACATATATTATTTCCAGAAAAGTAAAAAG
CCACTCTGGCAGGAGTGACTTTGAGCTTGATGTAAAAATCAAGTTTAATAAAAAACGACATTAA

Antitoxin

Nucleotide

• Length: 150 bp

>AT76651 NZ_CP021447:c1192159-1192010 [Clostridioides difficile]
GTAAATTCGTATCAAAAACAAAAAAGAAACTACAATCTATTAGCCTAGAGTGAAGTTTCATTAACTAAATATTAATGTCG
TTTTTTATTAAACTTGATTTTTACATCAAGCTCAAAGTCACTCCTGCCAGAGTGGCTTTTTACTTTTCTG

Structures

Toxin

• AlphaFold DB: A0A069A3R1

Antitoxin