Detailed information of TA system TA076645

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2606868..2607070
• Replicon: chromosome
• Accession: NZ_CP021445
• Organism: Clostridioides difficile strain DSM 104450

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: CDIF104450_RS12695
• Protein ID: WP_004454589.1
• Coordinates: 2606918..2607070 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	2606868..2606997 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF104450_RS12655 (CDIF104450_02542)	2601929..2602129	+	201	WP_021362723.1	hypothetical protein	-
CDIF104450_RS12660 (CDIF104450_02543)	2602507..2602824	+	318	WP_004454574.1	hypothetical protein	-
CDIF104450_RS12665 (CDIF104450_02544)	2603074..2603424	+	351	WP_021362724.1	hypothetical protein	-
CDIF104450_RS12670 (CDIF104450_02545)	2603753..2603914	-	162	WP_004454578.1	hypothetical protein	-
CDIF104450_RS12675 (CDIF104450_02546)	2603966..2604418	-	453	WP_021366820.1	hypothetical protein	-
CDIF104450_RS12680 (CDIF104450_02547)	2604415..2604780	-	366	WP_016729316.1	Bro-N domain-containing protein	-
CDIF104450_RS12685 (CDIF104450_02548)	2604901..2605089	-	189	WP_004454585.1	virulence factor Srl	-
CDIF104450_RS12690 (CDIF104450_02549)	2605802..2606650	-	849	WP_021366818.1	SHOCT domain-containing protein	-
-	2606868..2606997	+	130	-	-	Antitoxin
CDIF104450_RS12695 (CDIF104450_02550)	2606918..2607070	-	153	WP_004454589.1	hypothetical protein	Toxin
CDIF104450_RS12700 (CDIF104450_02551)	2607344..2607667	-	324	WP_021366819.1	DUF6054 family protein	-
CDIF104450_RS12705 (CDIF104450_02552)	2607703..2607972	-	270	WP_021366817.1	hypothetical protein	-
CDIF104450_RS12710	2608265..2608762	-	498	Protein_2428	transposase	-
CDIF104450_RS12715	2609052..2609427	-	376	Protein_2429	BlaI/MecI/CopY family transcriptional regulator	-
CDIF104450_RS12720 (CDIF104450_02555)	2609532..2609909	-	378	WP_021366893.1	BlaI/MecI/CopY family transcriptional regulator	-
CDIF104450_RS12725 (CDIF104450_02556)	2610714..2611208	+	495	WP_021364347.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
CDIF104450_RS12730	2611593..2611767	+	175	Protein_2432	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2599384..2617015	17631

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T76645 WP_004454589.1 NZ_CP021445:c2607070-2606918 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T76645 NZ_CP021445:c2607070-2606918 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACTGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT76645 NZ_CP021445:2606868-2606997 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCAGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin