Detailed information of TA system TA076640

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1752073..1752287
• Replicon: chromosome
• Accession: NZ_CP021445
• Organism: Clostridioides difficile strain DSM 104450

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: CDIF104450_RS08665
• Protein ID: WP_003429855.1
• Coordinates: 1752073..1752234 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1752154..1752287 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF104450_RS08620 (CDIF104450_01717)	1747398..1747631	+	234	WP_009896624.1	hypothetical protein	-
CDIF104450_RS08625 (CDIF104450_01718)	1747641..1748042	+	402	WP_021390278.1	hypothetical protein	-
CDIF104450_RS08630 (CDIF104450_01719)	1748036..1748398	+	363	WP_021390090.1	hypothetical protein	-
CDIF104450_RS08635 (CDIF104450_01720)	1748398..1748823	+	426	WP_021366369.1	HK97 gp10 family phage protein	-
CDIF104450_RS08640 (CDIF104450_01721)	1748816..1749253	+	438	WP_021366374.1	hypothetical protein	-
CDIF104450_RS08645 (CDIF104450_01722)	1749246..1749422	+	177	WP_016728451.1	hypothetical protein	-
CDIF104450_RS08650 (CDIF104450_01723)	1749423..1750733	+	1311	WP_021434813.1	phage tail sheath family protein	-
CDIF104450_RS08655 (CDIF104450_01724)	1750750..1751220	+	471	WP_003429851.1	phage tail tube protein	-
CDIF104450_RS08660 (CDIF104450_01725)	1751292..1751732	+	441	WP_003429853.1	phage portal protein	-
CDIF104450_RS08665	1752073..1752234	+	162	WP_003429855.1	hypothetical protein	Toxin
-	1752154..1752287	-	134	-	-	Antitoxin
CDIF104450_RS08670	1755171..1755359	+	189	WP_003429858.1	hypothetical protein	-
CDIF104450_RS08675 (CDIF104450_01727)	1755478..1756347	+	870	WP_021390102.1	BRO family protein	-
CDIF104450_RS08680 (CDIF104450_01728)	1756347..1756496	+	150	WP_021367044.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	erm(B)	-	1693390..1834317	140927

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T76640 WP_003429855.1 NZ_CP021445:1752073-1752234 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T76640 NZ_CP021445:1752073-1752234 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCATAAGTTCAAAT
AG

Antitoxin

Nucleotide

• Length: 134 bp

>AT76640 NZ_CP021445:c1752287-1752154 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin