Detailed information of TA system TA074672

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 3538762..3538971
• Replicon: chromosome
• Accession: NZ_CP020424
• Organism: Clostridioides difficile strain FDAARGOS_267

Toxin (Protein)

• Gene name: CD2517.1
• Uniprot ID: Q182K5
• Locus tag: A6J95_RS16525
• Protein ID: WP_004454815.1
• Coordinates: 3538762..3538920 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	3538837..3538971 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6J95_RS16495	3534558..3535226	+	669	WP_009897711.1	VanZ family protein	-
A6J95_RS16500	3535266..3535346	+	81	Protein_3103	VOC family protein	-
A6J95_RS16505	3535709..3535981	-	273	WP_004454820.1	tyrosine-type recombinase/integrase	-
A6J95_RS16510	3536212..3536633	-	422	Protein_3105	hypothetical protein	-
A6J95_RS16515	3536652..3536750	-	99	Protein_3106	Arm DNA-binding domain-containing protein	-
A6J95_RS16520	3537033..3538403	+	1371	WP_009897709.1	cell wall-binding protein Cwp29	-
A6J95_RS16525	3538762..3538920	+	159	WP_004454815.1	hypothetical protein	Toxin
-	3538837..3538971	-	135	NuclAT_0	-	Antitoxin
A6J95_RS16530	3540232..3540615	+	384	Protein_3109	BlaI/MecI/CopY family transcriptional regulator	-
A6J95_RS16535	3540804..3541799	-	996	WP_003431031.1	asparaginase	-
A6J95_RS16540	3541920..3543548	-	1629	WP_009897704.1	aspartate 4-decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (76-174)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5996.20 Da
• Isoelectric Point: 11.3102

>T74672 WP_004454815.1 NZ_CP020424:3538762-3538920 [Clostridioides difficile]
MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK

Nucleotide

• Length: 159 bp

>T74672 NZ_CP020424:3538762-3538920 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGCATACTAGCAAGTCTATCTGCTAGCTTAATAGTTTATATAGCTAGCAAACTATTTAGAAA
GCGTAAAAAACCACTCAAAGCGGCAACTAAGAGTGGTTGGGAATTTGATTTAAAAATCAGATTCCATAAAACTAAGTAA

Antitoxin

Nucleotide

• Length: 135 bp

>AT74672 NZ_CP020424:c3538971-3538837 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT

Structures

Toxin

• AlphaFold DB: A0A125V849

Antitoxin