Detailed information of TA system TA074668

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1898822..1899036
• Replicon: chromosome
• Accession: NZ_CP020424
• Organism: Clostridioides difficile strain FDAARGOS_267

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: A6J95_RS08840
• Protein ID: WP_003429855.1
• Coordinates: 1898875..1899036 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1898822..1898955 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6J95_RS08820	1895305..1896075	-	771	WP_018112719.1	SHOCT domain-containing protein	-
A6J95_RS20350	1896204..1896371	-	168	WP_018112720.1	hypothetical protein	-
A6J95_RS20355	1896426..1897253	-	828	WP_018112721.1	toxin Bro	-
A6J95_RS08835	1897377..1897550	-	174	WP_018112722.1	hypothetical protein	-
-	1898372..1898477	+	106	NuclAT_4	-	-
-	1898372..1898477	+	106	NuclAT_5	-	-
-	1898822..1898955	+	134	NuclAT_1	-	Antitoxin
-	1898822..1898955	+	134	NuclAT_2	-	Antitoxin
A6J95_RS08840	1898875..1899036	-	162	WP_003429855.1	hypothetical protein	Toxin
A6J95_RS08850	1899377..1899817	-	441	WP_018112723.1	phage portal protein	-
A6J95_RS08855	1899886..1900362	-	477	WP_018112724.1	phage tail tube protein	-
A6J95_RS08860	1900383..1901693	-	1311	WP_009896640.1	phage tail sheath family protein	-
A6J95_RS08865	1901694..1901870	-	177	WP_009896638.1	hypothetical protein	-
A6J95_RS08870	1901863..1902300	-	438	WP_018112725.1	hypothetical protein	-
A6J95_RS08875	1902293..1902718	-	426	WP_018112726.1	HK97 gp10 family phage protein	-
A6J95_RS08880	1902718..1903080	-	363	WP_018112727.1	hypothetical protein	-
A6J95_RS08885	1903080..1903424	-	345	WP_018112728.1	phage head-tail connector protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	groEL	1876777..1940361	63584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-85)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T74668 WP_003429855.1 NZ_CP020424:c1899036-1898875 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T74668 NZ_CP020424:c1899036-1898875 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCATAAGTTCAAAT
AG

Antitoxin

Nucleotide

• Length: 134 bp

>AT74668 NZ_CP020424:1898822-1898955 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin