Detailed information of TA system TA074482

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 299043..299246
• Replicon: chromosome
• Accession: NZ_CP020380
• Organism: Clostridioides difficile strain DSM 102978

Toxin (Protein)

• Gene name: CD2517.1
• Uniprot ID: -
• Locus tag: CDIF102978_RS20480
• Protein ID: WP_032508823.1
• Coordinates: 299043..299201 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	299120..299246 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF102978_RS01840	294321..294551	+	231	Protein_262	hypothetical protein	-
CDIF102978_RS01845	294624..295025	+	402	WP_022618353.1	hypothetical protein	-
CDIF102978_RS01850	295019..295366	+	348	WP_077709444.1	hypothetical protein	-
CDIF102978_RS01855	295366..295791	+	426	WP_003433542.1	HK97 gp10 family phage protein	-
CDIF102978_RS01860	295784..296221	+	438	WP_003433544.1	hypothetical protein	-
CDIF102978_RS01865	296214..296390	+	177	WP_003433548.1	hypothetical protein	-
CDIF102978_RS01870	296391..297701	+	1311	WP_003433550.1	phage tail sheath family protein	-
CDIF102978_RS01875	297718..298188	+	471	WP_021424215.1	phage tail tube protein	-
CDIF102978_RS01880	298260..298700	+	441	WP_003429853.1	phage portal protein	-
CDIF102978_RS20480	299043..299201	+	159	WP_032508823.1	hypothetical protein	Toxin
-	299120..299246	-	127	NuclAT_1	-	Antitoxin
-	299638..299744	-	107	NuclAT_2	-	-
CDIF102978_RS01890	299910..300119	-	210	WP_021424216.1	hypothetical protein	-
CDIF102978_RS01895	300766..301326	+	561	WP_021387709.1	ribosomal protein L7/L12	-
CDIF102978_RS01900	301402..303768	+	2367	WP_003433567.1	tape measure protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-120)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6104.39 Da
• Isoelectric Point: 11.5673

>T74482 WP_032508823.1 NZ_CP020380:299043-299201 [Clostridioides difficile]
MGNFLQNVLERLSVSLIVFLVSNLLKKRKKPLKAGTKSGWEFDLKIRFHKFK

Nucleotide

• Length: 159 bp

>T74482 NZ_CP020380:299043-299201 [Clostridioides difficile]
ATGGGTAATTTTTTACAAAATGTACTAGAAAGACTATCTGTTAGTTTAATAGTTTTTCTAGTTAGCAATTTACTTAAAAA
ACGTAAAAAACCACTCAAAGCTGGCACTAAGAGTGGTTGGGAATTTGATTTAAAAATCAGATTCCATAAGTTCAAATAA

Antitoxin

Nucleotide

• Length: 127 bp

>AT74482 NZ_CP020380:c299246-299120 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCAGTAGAGTGAAGTTCATAATACTTATTTGAACTTATGGAATCTGATTTTTAAATCAA
ATTCCCAACCACTCTTAGTGCCAGCTTTGAGTGGTTTTTTACGTTTT

Structures

Antitoxin