Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1083573..1084204 Replicon chromosome
Accession NZ_CP020342
Organism Shigella flexneri 1a strain 0439

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag BS647_RS05805 Protein ID WP_001259384.1
Coordinates 1083929..1084204 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag BS647_RS05800 Protein ID WP_000593555.1
Coordinates 1083573..1083932 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS647_RS05775 (1079705) 1079705..1080649 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
BS647_RS05780 (1080646) 1080646..1081479 + 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
BS647_RS05785 (1081479) 1081479..1082243 + 765 WP_001136253.1 nickel import ATP-binding protein NikD -
BS647_RS05790 (1082240) 1082240..1083046 + 807 WP_000173660.1 nickel import ATP-binding protein NikE -
BS647_RS05795 (1083052) 1083052..1083453 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
BS647_RS05800 (1083573) 1083573..1083932 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BS647_RS05805 (1083929) 1083929..1084204 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
BS647_RS05810 (1084263) 1084263..1085387 - 1125 WP_001216257.1 ABC transporter permease -
BS647_RS05815 (1085387) 1085387..1088122 - 2736 WP_000149170.1 ribosome-associated ATPase/putative transporter RbbA -
BS647_RS05820 (1088119) 1088119..1089186 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1083573..1103317 19744


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T74309 WP_001259384.1 NZ_CP020342:c1084204-1083929 [Shigella flexneri 1a]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T74309 NZ_CP020342:c1084204-1083929 [Shigella flexneri 1a]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT74309 WP_000593555.1 NZ_CP020342:c1083932-1083573 [Shigella flexneri 1a]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT74309 NZ_CP020342:c1083932-1083573 [Shigella flexneri 1a]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References