Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2903299..2903930 Replicon chromosome
Accession NZ_CP020339
Organism Shigella flexneri 4c strain 0702

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag BS653_RS16000 Protein ID WP_001259384.1
Coordinates 2903655..2903930 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag BS653_RS15995 Protein ID WP_000593555.1
Coordinates 2903299..2903658 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS653_RS15970 (2899431) 2899431..2900375 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
BS653_RS15975 (2900372) 2900372..2901205 + 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
BS653_RS15980 (2901205) 2901205..2901969 + 765 WP_001136253.1 nickel import ATP-binding protein NikD -
BS653_RS15985 (2901966) 2901966..2902772 + 807 WP_000173660.1 nickel import ATP-binding protein NikE -
BS653_RS15990 (2902778) 2902778..2903179 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
BS653_RS15995 (2903299) 2903299..2903658 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BS653_RS16000 (2903655) 2903655..2903930 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
BS653_RS16005 (2903989) 2903989..2905113 - 1125 WP_001216257.1 ABC transporter permease -
BS653_RS16010 (2905113) 2905113..2907848 - 2736 WP_000149170.1 ribosome-associated ATPase/putative transporter RbbA -
BS653_RS16015 (2907845) 2907845..2908912 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2903299..2923043 19744


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T74303 WP_001259384.1 NZ_CP020339:c2903930-2903655 [Shigella flexneri 4c]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T74303 NZ_CP020339:c2903930-2903655 [Shigella flexneri 4c]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT74303 WP_000593555.1 NZ_CP020339:c2903658-2903299 [Shigella flexneri 4c]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT74303 NZ_CP020339:c2903658-2903299 [Shigella flexneri 4c]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References