Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3734212..3734843 Replicon chromosome
Accession NZ_CP020336
Organism Shigella flexneri 4c strain 1602

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag BS654_RS20510 Protein ID WP_001259384.1
Coordinates 3734568..3734843 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag BS654_RS20505 Protein ID WP_000593555.1
Coordinates 3734212..3734571 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS654_RS20480 (3730344) 3730344..3731288 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
BS654_RS20485 (3731285) 3731285..3732118 + 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
BS654_RS20490 (3732118) 3732118..3732882 + 765 WP_001136253.1 nickel import ATP-binding protein NikD -
BS654_RS20495 (3732879) 3732879..3733685 + 807 WP_000173660.1 nickel import ATP-binding protein NikE -
BS654_RS20500 (3733691) 3733691..3734092 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
BS654_RS20505 (3734212) 3734212..3734571 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BS654_RS20510 (3734568) 3734568..3734843 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
BS654_RS20515 (3734902) 3734902..3736026 - 1125 WP_094085517.1 ABC transporter permease -
BS654_RS20520 (3736026) 3736026..3738761 - 2736 WP_000149170.1 ribosome-associated ATPase/putative transporter RbbA -
BS654_RS20525 (3738758) 3738758..3739825 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3734212..3753956 19744


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T74280 WP_001259384.1 NZ_CP020336:c3734843-3734568 [Shigella flexneri 4c]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T74280 NZ_CP020336:c3734843-3734568 [Shigella flexneri 4c]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT74280 WP_000593555.1 NZ_CP020336:c3734571-3734212 [Shigella flexneri 4c]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT74280 NZ_CP020336:c3734571-3734212 [Shigella flexneri 4c]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References