Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 824061..824692 Replicon chromosome
Accession NZ_CP020086
Organism Shigella flexneri 1a strain 0670

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag B4376_RS04190 Protein ID WP_001259384.1
Coordinates 824061..824336 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag B4376_RS04195 Protein ID WP_000593555.1
Coordinates 824333..824692 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4376_RS04175 (819079) 819079..820146 + 1068 WP_000361470.1 HlyD family secretion protein -
B4376_RS04180 (820143) 820143..822878 + 2736 WP_000149170.1 ribosome-associated ATPase/putative transporter RbbA -
B4376_RS04185 (822878) 822878..824002 + 1125 WP_001216257.1 ABC transporter permease -
B4376_RS04190 (824061) 824061..824336 + 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
B4376_RS04195 (824333) 824333..824692 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
B4376_RS04200 (824812) 824812..825213 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
B4376_RS04205 (825219) 825219..826025 - 807 WP_000173660.1 nickel import ATP-binding protein NikE -
B4376_RS04210 (826022) 826022..826786 - 765 WP_001136253.1 nickel import ATP-binding protein NikD -
B4376_RS04215 (826786) 826786..827619 - 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
B4376_RS04220 (827616) 827616..828560 - 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 803674..824692 21018


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T74001 WP_001259384.1 NZ_CP020086:824061-824336 [Shigella flexneri 1a]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T74001 NZ_CP020086:824061-824336 [Shigella flexneri 1a]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT74001 WP_000593555.1 NZ_CP020086:824333-824692 [Shigella flexneri 1a]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT74001 NZ_CP020086:824333-824692 [Shigella flexneri 1a]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References