Detailed information of TA system TA073396

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1499311..1499513
• Replicon: chromosome
• Accession: NZ_CP019870
• Organism: Clostridioides difficile strain BR81

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: BZ168_RS06490
• Protein ID: WP_004454589.1
• Coordinates: 1499311..1499463 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	1499384..1499513 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BZ168_RS18965	1494478..1494648	-	171	WP_004454601.1	hypothetical protein	-
BZ168_RS06460	1495037..1495525	-	489	WP_004454599.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
BZ168_RS06465	1496331..1496705	+	375	WP_004454597.1	BlaI/MecI/CopY family transcriptional regulator	-
BZ168_RS18970	1496810..1497184	+	375	Protein_1272	BlaI/MecI/CopY family transcriptional regulator	-
BZ168_RS06475	1497474..1497992	+	519	Protein_1273	transposase	-
BZ168_RS06480	1498424..1498678	+	255	WP_004454592.1	hypothetical protein	-
BZ168_RS06485	1498714..1499037	+	324	WP_009897482.1	hypothetical protein	-
BZ168_RS06490	1499311..1499463	+	153	WP_004454589.1	hypothetical protein	Toxin
-	1499384..1499513	-	130	NuclAT_1	-	Antitoxin
BZ168_RS06495	1499657..1500580	+	924	WP_004454587.1	SHOCT domain-containing protein	-
BZ168_RS19170	1502117..1502269	+	153	WP_009897477.1	hypothetical protein	-
BZ168_RS19175	1502390..1503203	+	814	Protein_1279	toxin Bro	-
BZ168_RS06510	1503255..1503416	+	162	WP_004454578.1	hypothetical protein	-
BZ168_RS06520	1503745..1504209	-	465	WP_004454576.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1434060..1514622	80562

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T73396 WP_004454589.1 NZ_CP019870:1499311-1499463 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T73396 NZ_CP019870:1499311-1499463 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT73396 NZ_CP019870:c1499513-1499384 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin