Detailed information of TA system TA073382

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 3654523..3654738
• Replicon: chromosome
• Accession: NZ_CP019860
• Organism: Clostridioides difficile strain DSM 29632

Toxin (Protein)

• Gene name: CD2889
• Uniprot ID: Q183X5
• Locus tag: CDIF29632_RS16855
• Protein ID: WP_011861731.1
• Coordinates: 3654595..3654738 (-)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	RCd12
Locus tag	-
Coordinates	3654523..3654669 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF29632_RS16825	3649746..3650570	-	825	WP_003432350.1	phosphate ABC transporter permease PstA	-
CDIF29632_RS16830	3650572..3651489	-	918	WP_021361136.1	phosphate ABC transporter permease subunit PstC	-
CDIF29632_RS16835	3651870..3652316	-	447	WP_167652391.1	hypothetical protein	-
CDIF29632_RS16840	3653039..3653425	-	387	WP_021366416.1	BlaI/MecI/CopY family transcriptional regulator	-
CDIF29632_RS16845	3653465..3653746	-	282	WP_070538156.1	BlaI/MecI/CopY family transcriptional regulator	-
CDIF29632_RS16850	3654214..3654390	+	177	WP_021358767.1	hypothetical protein	-
-	3654523..3654669	+	147	NuclAT_0	-	Antitoxin
-	3654523..3654673	+	151	NuclAT_1	-	-
CDIF29632_RS16855	3654595..3654738	-	144	WP_011861731.1	hypothetical protein	Toxin
CDIF29632_RS16860	3654996..3655184	+	189	WP_021360368.1	hypothetical protein	-
CDIF29632_RS16865	3655779..3656807	-	1029	WP_032544344.1	hypothetical protein	-
CDIF29632_RS16870	3657220..3658041	-	822	WP_077770701.1	N-acetylmuramoyl-L-alanine amidase	-
CDIF29632_RS16875	3658041..3658532	-	492	WP_077770700.1	phage holin family protein	-
CDIF29632_RS16880	3658540..3658758	-	219	WP_009894435.1	hypothetical protein	-
CDIF29632_RS16885	3658858..3659040	-	183	WP_070537881.1	hypothetical protein	-
CDIF29632_RS16890	3659040..3659336	-	297	WP_070537883.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3648963..3703687	54724

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-116)

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5399.34 Da
• Isoelectric Point: 10.8277

>T73382 WP_011861731.1 NZ_CP019860:c3654738-3654595 [Clostridioides difficile]
MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNKKRH

Nucleotide

• Length: 144 bp

>T73382 NZ_CP019860:c3654738-3654595 [Clostridioides difficile]
ATGAGCGAATTTTTACTAGGAGTGTTAGCTAGTTTAACAGCTAGCTTTATTACATATATTATTTCCAGAAAAGTAAAAAG
CCACTCTGGCAGGAGTGACTTTGAGCTTGATGTAAAAATCAAGTTTAATAAAAAACGACATTAA

Antitoxin

Nucleotide

• Length: 147 bp

>AT73382 NZ_CP019860:3654523-3654669 [Clostridioides difficile]
GTAAATTCGTATCAAAAACAAAAAAAGAAACTACAATCTATTAGCCTAGAGTGAAGTTTCATTAACTAAATATTAATGTC
GTTTTTTATTAAACTTGATTTTTACATCAAGCTCAAAGTCACTCCTGCCAGAGTGGCTTTTTACTTT

Structures

Toxin

• AlphaFold DB: A0A069A3R1

Antitoxin