Detailed information of TA system TA073376

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2515324..2515526
• Replicon: chromosome
• Accession: NZ_CP019860
• Organism: Clostridioides difficile strain DSM 29632

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: CDIF29632_RS11990
• Protein ID: WP_004454589.1
• Coordinates: 2515374..2515526 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	2515324..2515453 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF29632_RS11955	2510639..2510956	+	318	WP_004454574.1	hypothetical protein	-
CDIF29632_RS11960	2511092..2511556	+	465	WP_021365574.1	hypothetical protein	-
CDIF29632_RS11970	2511880..2512041	-	162	WP_004454578.1	hypothetical protein	-
CDIF29632_RS11975	2512093..2512545	-	453	WP_021422473.1	hypothetical protein	-
CDIF29632_RS11980	2512542..2512907	-	366	WP_016729316.1	Bro-N domain-containing protein	-
CDIF29632_RS19560	2513028..2513180	-	153	WP_009897477.1	hypothetical protein	-
CDIF29632_RS11985	2514296..2515180	-	885	WP_009897481.1	hypothetical protein	-
-	2515324..2515453	+	130	NuclAT_5	-	Antitoxin
CDIF29632_RS11990	2515374..2515526	-	153	WP_004454589.1	hypothetical protein	Toxin
CDIF29632_RS11995	2515800..2516123	-	324	WP_009897482.1	hypothetical protein	-
CDIF29632_RS12000	2516159..2516413	-	255	WP_004454592.1	hypothetical protein	-
CDIF29632_RS12010	2516845..2517363	-	519	Protein_2220	transposase	-
CDIF29632_RS12015	2517653..2518027	-	375	Protein_2221	BlaI/MecI/CopY family transcriptional regulator	-
CDIF29632_RS12020	2518132..2518506	-	375	WP_004454597.1	BlaI/MecI/CopY family transcriptional regulator	-
CDIF29632_RS12025	2519310..2519798	+	489	WP_004454599.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
CDIF29632_RS12030	2520187..2520357	+	171	WP_021394397.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2508819..2525606	16787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-134)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T73376 WP_004454589.1 NZ_CP019860:c2515526-2515374 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T73376 NZ_CP019860:c2515526-2515374 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT73376 NZ_CP019860:2515324-2515453 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin