Detailed information of TA system TA073372

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1706484..1706698
• Replicon: chromosome
• Accession: NZ_CP019860
• Organism: Clostridioides difficile strain DSM 29632

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: CDIF29632_RS08190
• Protein ID: WP_003429855.1
• Coordinates: 1706484..1706645 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1706565..1706698 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF29632_RS08140	1701824..1702057	+	234	WP_003433536.1	hypothetical protein	-
CDIF29632_RS08145	1702067..1702468	+	402	WP_009888836.1	hypothetical protein	-
CDIF29632_RS08150	1702462..1702809	+	348	WP_003433540.1	hypothetical protein	-
CDIF29632_RS08155	1702809..1703234	+	426	WP_003433542.1	HK97 gp10 family phage protein	-
CDIF29632_RS08160	1703227..1703664	+	438	WP_003433544.1	hypothetical protein	-
CDIF29632_RS08165	1703657..1703833	+	177	WP_003433548.1	hypothetical protein	-
CDIF29632_RS08170	1703834..1705144	+	1311	WP_003433550.1	phage tail sheath family protein	-
CDIF29632_RS08175	1705161..1705631	+	471	WP_003429851.1	phage tail tube protein	-
CDIF29632_RS08180	1705703..1706143	+	441	WP_003429853.1	phage portal protein	-
CDIF29632_RS08190	1706484..1706645	+	162	WP_003429855.1	hypothetical protein	Toxin
-	1706565..1706698	-	134	NuclAT_3	-	Antitoxin
-	1706565..1706698	-	134	NuclAT_4	-	Antitoxin
-	1707043..1707091	-	49	NuclAT_8	-	-
-	1707043..1707091	-	49	NuclAT_9	-	-
CDIF29632_RS08195	1707970..1708545	+	576	WP_043897361.1	SHOCT domain-containing protein	-
CDIF29632_RS08200	1708849..1709022	+	174	WP_003433557.1	hypothetical protein	-
CDIF29632_RS19540	1709146..1709973	+	828	WP_003433559.1	hypothetical protein	-
CDIF29632_RS19545	1710022..1710198	+	177	WP_003433561.1	hypothetical protein	-
CDIF29632_RS08210	1710629..1711189	+	561	WP_021387709.1	ribosomal protein L7/L12	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1672806..1737865	65059

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T73372 WP_003429855.1 NZ_CP019860:1706484-1706645 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T73372 NZ_CP019860:1706484-1706645 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCATAAGTTCAAAT
AG

Antitoxin

Nucleotide

• Length: 134 bp

>AT73372 NZ_CP019860:c1706698-1706565 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin