Detailed information of TA system TA073361

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2606687..2606889
• Replicon: chromosome
• Accession: NZ_CP019858
• Organism: Clostridioides difficile strain DSM 29688

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: CDIF29688_RS12975
• Protein ID: WP_004454589.1
• Coordinates: 2606737..2606889 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	2606687..2606816 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF29688_RS12935	2601748..2601948	+	201	WP_021362723.1	hypothetical protein	-
CDIF29688_RS12940	2602326..2602643	+	318	WP_004454574.1	hypothetical protein	-
CDIF29688_RS12945	2602779..2603243	+	465	WP_025782691.1	hypothetical protein	-
CDIF29688_RS12955	2603572..2603733	-	162	WP_004454578.1	hypothetical protein	-
CDIF29688_RS12960	2603785..2604237	-	453	WP_021366820.1	hypothetical protein	-
CDIF29688_RS12965	2604234..2604599	-	366	WP_016729316.1	Bro-N domain-containing protein	-
CDIF29688_RS20325	2604720..2604872	-	153	WP_009897477.1	hypothetical protein	-
CDIF29688_RS12970	2605621..2606543	-	923	Protein_2408	SHOCT domain-containing protein	-
-	2606687..2606816	+	130	NuclAT_7	-	Antitoxin
CDIF29688_RS12975	2606737..2606889	-	153	WP_004454589.1	hypothetical protein	Toxin
CDIF29688_RS12980	2607163..2607486	-	324	WP_021366819.1	hypothetical protein	-
CDIF29688_RS12985	2607522..2607776	-	255	WP_025782692.1	hypothetical protein	-
CDIF29688_RS12990	2608084..2608581	-	498	Protein_2412	transposase	-
CDIF29688_RS12995	2608871..2609246	-	376	Protein_2413	BlaI/MecI/CopY family transcriptional regulator	-
CDIF29688_RS13000	2609351..2609728	-	378	WP_021366893.1	BlaI/MecI/CopY family transcriptional regulator	-
CDIF29688_RS13005	2610533..2611027	+	495	WP_021364347.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
CDIF29688_RS13010	2611416..2611586	+	171	WP_032509469.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2599203..2615336	16133

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T73361 WP_004454589.1 NZ_CP019858:c2606889-2606737 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T73361 NZ_CP019858:c2606889-2606737 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACTGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT73361 NZ_CP019858:2606687-2606816 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCAGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin