Detailed information of TA system TA073357

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1752041..1752255
• Replicon: chromosome
• Accession: NZ_CP019858
• Organism: Clostridioides difficile strain DSM 29688

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: CDIF29688_RS08830
• Protein ID: WP_003429855.1
• Coordinates: 1752041..1752202 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1752122..1752255 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF29688_RS08780	1747366..1747599	+	234	WP_009896624.1	hypothetical protein	-
CDIF29688_RS08785	1747609..1748010	+	402	WP_021390278.1	hypothetical protein	-
CDIF29688_RS08790	1748004..1748366	+	363	WP_021390090.1	hypothetical protein	-
CDIF29688_RS08795	1748366..1748791	+	426	WP_021366369.1	HK97 gp10 family phage protein	-
CDIF29688_RS08800	1748784..1749221	+	438	WP_021366374.1	hypothetical protein	-
CDIF29688_RS08805	1749214..1749390	+	177	WP_016728451.1	hypothetical protein	-
CDIF29688_RS08810	1749391..1750701	+	1311	WP_021434813.1	phage tail sheath family protein	-
CDIF29688_RS08815	1750718..1751188	+	471	WP_003429851.1	phage tail tube protein	-
CDIF29688_RS08820	1751260..1751700	+	441	WP_003429853.1	phage portal protein	-
CDIF29688_RS08830	1752041..1752202	+	162	WP_003429855.1	hypothetical protein	Toxin
-	1752122..1752255	-	134	NuclAT_2	-	Antitoxin
-	1752122..1752255	-	134	NuclAT_4	-	Antitoxin
-	1752600..1752648	-	49	NuclAT_11	-	-
-	1752600..1752648	-	49	NuclAT_13	-	-
-	1753755..1753860	-	106	NuclAT_10	-	-
-	1753755..1753860	-	106	NuclAT_12	-	-
CDIF29688_RS08835	1755139..1755327	+	189	WP_003429858.1	hypothetical protein	-
CDIF29688_RS08840	1755446..1756315	+	870	WP_021390102.1	hypothetical protein	-
CDIF29688_RS20240	1756315..1756464	+	150	WP_021367044.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	erm(B)	-	1693357..1834285	140928

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T73357 WP_003429855.1 NZ_CP019858:1752041-1752202 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T73357 NZ_CP019858:1752041-1752202 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCATAAGTTCAAAT
AG

Antitoxin

Nucleotide

• Length: 134 bp

>AT73357 NZ_CP019858:c1752255-1752122 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin