Detailed information of TA system TA072528

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 3180330..3180544
• Replicon: chromosome
• Accession: NZ_CP019469
• Organism: Clostridioides difficile strain LEM1

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: BWD37_RS15540
• Protein ID: WP_003429855.1
• Coordinates: 3180383..3180544 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	3180330..3180463 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWD37_RS15510 (3175877)	3175877..3176029	-	153	WP_021378619.1	hypothetical protein	-
BWD37_RS15515 (3176062)	3176062..3176841	-	780	WP_021391616.1	ORF6N domain-containing protein	-
BWD37_RS15520 (3177116)	3177116..3177328	+	213	WP_016728883.1	helix-turn-helix transcriptional regulator	-
BWD37_RS15525 (3177747)	3177747..3177902	-	156	WP_021378513.1	hypothetical protein	-
BWD37_RS15530 (3177954)	3177954..3178820	-	867	WP_021378510.1	BRO family protein	-
BWD37_RS15535 (3178939)	3178939..3179127	-	189	WP_003429858.1	hypothetical protein	-
- (3179881)	3179881..3179986	+	106	NuclAT_11	-	-
- (3179881)	3179881..3179986	+	106	NuclAT_6	-	-
- (3179881)	3179881..3179986	+	106	NuclAT_6	-	-
- (3179881)	3179881..3179986	+	106	NuclAT_9	-	-
- (3180330)	3180330..3180463	+	134	NuclAT_3	-	Antitoxin
- (3180330)	3180330..3180463	+	134	NuclAT_3	-	Antitoxin
- (3180330)	3180330..3180463	+	134	NuclAT_4	-	Antitoxin
- (3180330)	3180330..3180463	+	134	NuclAT_6	-	Antitoxin
BWD37_RS15540 (3180383)	3180383..3180544	-	162	WP_003429855.1	hypothetical protein	Toxin
BWD37_RS15545 (3180885)	3180885..3181325	-	441	WP_003429853.1	phage portal protein	-
BWD37_RS15550 (3181397)	3181397..3181867	-	471	WP_003429851.1	phage tail tube protein	-
BWD37_RS15555 (3181884)	3181884..3183194	-	1311	WP_021368245.1	phage tail sheath family protein	-
BWD37_RS15560 (3183196)	3183196..3183372	-	177	WP_021362376.1	hypothetical protein	-
BWD37_RS15565 (3183365)	3183365..3183562	-	198	WP_021400151.1	hypothetical protein	-
BWD37_RS15570 (3183577)	3183577..3183801	-	225	WP_261799548.1	hypothetical protein	-
BWD37_RS15575 (3183794)	3183794..3184219	-	426	WP_003429845.1	HK97 gp10 family phage protein	-
BWD37_RS15580 (3184219)	3184219..3184566	-	348	WP_003429844.1	hypothetical protein	-
BWD37_RS15585 (3184560)	3184560..3184961	-	402	WP_021379034.1	hypothetical protein	-
BWD37_RS15590 (3184971)	3184971..3185204	-	234	WP_003429842.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T72528 WP_003429855.1 NZ_CP019469:c3180544-3180383 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T72528 NZ_CP019469:c3180544-3180383 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCATAAGTTCAAAT
AG

Antitoxin

Nucleotide

• Length: 134 bp

>AT72528 NZ_CP019469:3180330-3180463 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin