Detailed information of TA system TA072524

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 3156688..3156903
• Replicon: chromosome
• Accession: NZ_CP019469
• Organism: Clostridioides difficile strain LEM1

Toxin (Protein)

• Gene name: CD2889
• Uniprot ID: Q183X5
• Locus tag: BWD37_RS15380
• Protein ID: WP_011861731.1
• Coordinates: 3156760..3156903 (-)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	RCd12
Locus tag	-
Coordinates	3156688..3156834 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWD37_RS15345 (3152183)	3152183..3152941	-	759	WP_009888882.1	PTS sugar transporter subunit IIC	-
BWD37_RS15350 (3152958)	3152958..3153428	-	471	WP_003428544.1	PTS sugar transporter subunit IIB	-
BWD37_RS15355 (3153428)	3153428..3154171	-	744	WP_003437997.1	BtpA/SgcQ family protein	-
BWD37_RS15360 (3154175)	3154175..3154588	-	414	WP_009896035.1	PTS sugar transporter subunit IIA	-
BWD37_RS15365 (3154614)	3154614..3155189	-	576	WP_021360401.1	GntR family transcriptional regulator	-
BWD37_RS15370 (3155383)	3155383..3155766	-	384	WP_015984867.1	BlaI/MecI/CopY family transcriptional regulator	-
BWD37_RS15375 (3155876)	3155876..3156253	-	378	WP_021391346.1	BlaI/MecI/CopY family transcriptional regulator	-
- (3156688)	3156688..3156834	+	147	NuclAT_0	-	Antitoxin
- (3156688)	3156688..3156834	+	147	NuclAT_0	-	Antitoxin
- (3156688)	3156688..3156834	+	147	NuclAT_0	-	Antitoxin
- (3156688)	3156688..3156838	+	151	NuclAT_1	-	-
BWD37_RS15380 (3156760)	3156760..3156903	-	144	WP_011861731.1	hypothetical protein	Toxin
BWD37_RS15385 (3157161)	3157161..3157349	+	189	WP_009898401.1	hypothetical protein	-
BWD37_RS15390 (3157759)	3157759..3158325	-	567	WP_016056345.1	hypothetical protein	-
BWD37_RS15395 (3158327)	3158327..3158818	-	492	WP_016056344.1	hypothetical protein	-
BWD37_RS15400 (3159068)	3159068..3159880	-	813	WP_016056343.1	N-acetylmuramoyl-L-alanine amidase	-
BWD37_RS15405 (3159880)	3159880..3160137	-	258	WP_009898413.1	phage holin family protein	-
BWD37_RS15410 (3160157)	3160157..3160387	-	231	WP_021364192.1	hemolysin XhlA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5399.34 Da
• Isoelectric Point: 10.8277

>T72524 WP_011861731.1 NZ_CP019469:c3156903-3156760 [Clostridioides difficile]
MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNKKRH

Nucleotide

• Length: 144 bp

>T72524 NZ_CP019469:c3156903-3156760 [Clostridioides difficile]
ATGAGCGAATTTTTACTAGGAGTGTTAGCTAGTTTAACAGCTAGCTTTATTACATATATTATTTCCAGAAAAGTAAAAAG
CCACTCTGGCAGGAGTGACTTTGAGCTTGATGTAAAAATCAAGTTTAATAAAAAACGACATTAA

Antitoxin

Nucleotide

• Length: 147 bp

>AT72524 NZ_CP019469:3156688-3156834 [Clostridioides difficile]
GTAAATTCGTATCAAAAACAAAAAAAGAAACTACAATCTATTAGCCTAGAGTGAAGTTTCATTAACTAAATATTAATGTC
GTTTTTTATTAAACTTGATTTTTACATCAAGCTCAAAGTCACTCCTGCCAGAGTGGCTTTTTACTTT

Structures

Toxin

• AlphaFold DB: A0A069A3R1

Antitoxin