Detailed information of TA system TA072520

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2618950..2619164
• Replicon: chromosome
• Accession: NZ_CP019469
• Organism: Clostridioides difficile strain LEM1

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: BWD37_RS12575
• Protein ID: WP_003429855.1
• Coordinates: 2619003..2619164 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	2618950..2619083 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWD37_RS12550 (2614653)	2614653..2615456	-	804	WP_021377655.1	ORF6N domain-containing protein	-
BWD37_RS12555 (2615700)	2615700..2615903	+	204	WP_021367646.1	helix-turn-helix transcriptional regulator	-
BWD37_RS12560 (2616316)	2616316..2616492	-	177	WP_009894378.1	hypothetical protein	-
BWD37_RS12565 (2616555)	2616555..2617382	-	828	WP_021368246.1	hypothetical protein	-
BWD37_RS12570 (2617506)	2617506..2617679	-	174	WP_018112722.1	hypothetical protein	-
- (2618501)	2618501..2618606	+	106	NuclAT_10	-	-
- (2618501)	2618501..2618606	+	106	NuclAT_5	-	-
- (2618501)	2618501..2618606	+	106	NuclAT_5	-	-
- (2618501)	2618501..2618606	+	106	NuclAT_8	-	-
- (2618950)	2618950..2619083	+	134	NuclAT_2	-	Antitoxin
- (2618950)	2618950..2619083	+	134	NuclAT_2	-	Antitoxin
- (2618950)	2618950..2619083	+	134	NuclAT_3	-	Antitoxin
- (2618950)	2618950..2619083	+	134	NuclAT_5	-	Antitoxin
BWD37_RS12575 (2619003)	2619003..2619164	-	162	WP_003429855.1	hypothetical protein	Toxin
BWD37_RS12580 (2619505)	2619505..2619945	-	441	WP_003429853.1	phage portal protein	-
BWD37_RS12585 (2620017)	2620017..2620487	-	471	WP_003429851.1	phage tail tube protein	-
BWD37_RS12590 (2620504)	2620504..2621814	-	1311	WP_021368245.1	phage tail sheath family protein	-
BWD37_RS12595 (2621816)	2621816..2621992	-	177	WP_021362376.1	hypothetical protein	-
BWD37_RS12600 (2621985)	2621985..2622422	-	438	WP_021362375.1	hypothetical protein	-
BWD37_RS12605 (2622771)	2622771..2623577	-	807	WP_021358994.1	hypothetical protein	-
BWD37_RS12610 (2623687)	2623687..2623977	-	291	WP_021358995.1	HK97 gp10 family phage protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T72520 WP_003429855.1 NZ_CP019469:c2619164-2619003 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T72520 NZ_CP019469:c2619164-2619003 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCATAAGTTCAAAT
AG

Antitoxin

Nucleotide

• Length: 134 bp

>AT72520 NZ_CP019469:2618950-2619083 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin