Detailed information of TA system TA072517

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1750419..1750621
• Replicon: chromosome
• Accession: NZ_CP019469
• Organism: Clostridioides difficile strain LEM1

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: BWD37_RS08370
• Protein ID: WP_004454589.1
• Coordinates: 1750419..1750571 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	1750492..1750621 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWD37_RS08345 (1746140)	1746140..1746634	-	495	WP_021364347.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
BWD37_RS08350 (1747919)	1747919..1748110	+	192	WP_021364351.1	BlaI/MecI/CopY family transcriptional regulator	-
BWD37_RS08355 (1748583)	1748583..1749101	+	519	Protein_1562	transposase	-
BWD37_RS08360 (1749533)	1749533..1749787	+	255	WP_004454592.1	hypothetical protein	-
BWD37_RS08365 (1749823)	1749823..1750158	+	336	WP_021398462.1	DUF6054 family protein	-
BWD37_RS08370 (1750419)	1750419..1750571	+	153	WP_004454589.1	hypothetical protein	Toxin
- (1750492)	1750492..1750621	-	130	NuclAT_4	-	Antitoxin
- (1750492)	1750492..1750621	-	130	NuclAT_4	-	Antitoxin
- (1750492)	1750492..1750621	-	130	NuclAT_7	-	Antitoxin
BWD37_RS08375 (1750839)	1750839..1751369	+	531	WP_261799965.1	hypothetical protein	-
BWD37_RS08380 (1751582)	1751582..1751686	+	105	WP_231299121.1	SHOCT domain-containing protein	-
BWD37_RS08385 (1752824)	1752824..1752976	+	153	WP_009897477.1	hypothetical protein	-
BWD37_RS08390 (1753096)	1753096..1753461	+	366	WP_009897471.1	Bro-N domain-containing protein	-
BWD37_RS08395 (1753458)	1753458..1753910	+	453	WP_009897470.1	hypothetical protein	-
BWD37_RS08400 (1753961)	1753961..1754122	+	162	WP_004454578.1	hypothetical protein	-
BWD37_RS08405 (1754252)	1754252..1754422	+	171	Protein_1572	cell wall-binding repeat-containing protein	-
BWD37_RS08410 (1754451)	1754451..1754915	-	465	WP_004454576.1	hypothetical protein	-
BWD37_RS08415 (1755051)	1755051..1755368	-	318	WP_009897465.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T72517 WP_004454589.1 NZ_CP019469:1750419-1750571 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T72517 NZ_CP019469:1750419-1750571 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT72517 NZ_CP019469:c1750621-1750492 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin