Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) symE-ohsC/SymE(toxin)
Location 4521486..4521898 Replicon chromosome
Accession NZ_CP017100
Organism Escherichia coli strain K-12 NEB 5-alpha

Toxin (Protein)


Gene name symE Uniprot ID U9YSY7
Locus tag NEB5A_RS22415 Protein ID WP_000132601.1
Coordinates 4521486..4521827 (-) Length 114 a.a.

Antitoxin (RNA)


Gene name ohsC
Locus tag -
Coordinates 4521822..4521898 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NEB5A_RS22405 4518899..4519945 - 1047 WP_000437621.1 5-methylcytosine-specific restriction endonuclease system specificity protein McrC -
NEB5A_RS22410 4519945..4521324 - 1380 WP_000443951.1 5-methylcytosine-specific restriction endonuclease subunit McrB -
NEB5A_RS22415 4521486..4521827 - 342 WP_000132601.1 endoribonuclease SymE Toxin
- 4521822..4521898 + 77 NuclAT_14 - Antitoxin
- 4521822..4521898 + 77 NuclAT_14 - Antitoxin
- 4521822..4521898 + 77 NuclAT_14 - Antitoxin
- 4521822..4521898 + 77 NuclAT_14 - Antitoxin
- 4521822..4521898 + 77 NuclAT_15 - Antitoxin
- 4521822..4521898 + 77 NuclAT_15 - Antitoxin
- 4521822..4521898 + 77 NuclAT_15 - Antitoxin
- 4521822..4521898 + 77 NuclAT_15 - Antitoxin
NEB5A_RS22420 4522055..4523449 - 1395 WP_001272447.1 type I restriction-modification system specificity subunit -
NEB5A_RS22425 4523446..4525035 - 1590 WP_001063204.1 type I restriction-modification system methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4514401..4523449 9048


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SymE-like

(20-71)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12203.02 Da        Isoelectric Point: 8.5012

>T66001 WP_000132601.1 NZ_CP017100:c4521827-4521486 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Download         Length: 342 bp

>T66001 NZ_CP017100:c4521827-4521486 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin


Download         Length: 77 bp

>AT66001 NZ_CP017100:4521822-4521898 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YSY7

Antitoxin

Download structure file

References