Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1591230..1591756 Replicon chromosome
Accession NZ_CP017100
Organism Escherichia coli strain K-12 NEB 5-alpha

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag NEB5A_RS07995 Protein ID WP_000323025.1
Coordinates 1591230..1591517 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag NEB5A_RS08000 Protein ID WP_000534858.1
Coordinates 1591517..1591756 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NEB5A_RS07955 1586254..1586469 - 216 WP_000839590.1 phage lysis protein EssD -
NEB5A_RS25595 1586689..1586859 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
NEB5A_RS07960 1587223..1587438 - 216 WP_000066484.1 cold shock-like protein CspB -
NEB5A_RS07965 1587739..1587951 + 213 WP_000087756.1 cold shock-like protein CspF -
NEB5A_RS25175 1588006..1588095 + 90 WP_120795389.1 hypothetical protein -
NEB5A_RS07970 1588373..1589125 - 753 WP_001047135.1 antitermination protein -
NEB5A_RS07975 1589139..1590188 - 1050 WP_001265198.1 DUF968 domain-containing protein -
NEB5A_RS07980 1590190..1590468 - 279 WP_012304870.1 hypothetical protein -
NEB5A_RS07985 1590535..1590786 - 252 WP_000980994.1 hypothetical protein -
NEB5A_RS07990 1591003..1591158 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
NEB5A_RS07995 1591230..1591517 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
NEB5A_RS08000 1591517..1591756 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
NEB5A_RS23390 1591781..1592086 + 306 WP_001326990.1 hypothetical protein -
NEB5A_RS08005 1592289..1592621 + 333 WP_001301033.1 protein FlxA -
NEB5A_RS08010 1593058..1593207 - 150 WP_011443592.1 hypothetical protein -
NEB5A_RS08015 1593242..1593520 - 279 Protein_1539 hypothetical protein -
NEB5A_RS08020 1593504..1593734 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
NEB5A_RS08025 1593818..1594225 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
NEB5A_RS08030 1594392..1594547 + 156 WP_000379575.1 DUF1391 family protein -
NEB5A_RS08035 1594707..1594925 + 219 WP_001171942.1 hypothetical protein -
NEB5A_RS08045 1595493..1595681 + 189 WP_000854559.1 cell division inhibition protein DicB -
NEB5A_RS08050 1595678..1595869 + 192 WP_001083297.1 lysis protein YdfD -
NEB5A_RS23405 1595962..1596729 + 768 Protein_1546 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1576886..1599799 22913


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T65978 WP_000323025.1 NZ_CP017100:c1591517-1591230 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T65978 NZ_CP017100:c1591517-1591230 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT65978 WP_000534858.1 NZ_CP017100:c1591756-1591517 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT65978 NZ_CP017100:c1591756-1591517 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References