Detailed information of TA system TA064415

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 3337426..3337641
• Replicon: chromosome
• Accession: NZ_CP016318
• Organism: Clostridioides difficile strain 630 delta erm

Toxin (Protein)

• Gene name: CD2889
• Uniprot ID: Q183X5
• Locus tag: CDIF630erm_RS20760
• Protein ID: WP_011861731.1
• Coordinates: 3337498..3337641 (-)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	3337426..3337572 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF630erm_RS15700	3333085..3334239	+	1155	WP_003439713.1	GGDEF domain-containing protein	-
CDIF630erm_RS15705	3334320..3336038	-	1719	WP_011861730.1	putative 2-aminoethylphosphonate ABC transporter permease subunit	-
CDIF630erm_RS15710	3336043..3336924	-	882	Protein_2956	ATP-binding cassette domain-containing protein	-
-	3337426..3337572	+	147	NuclAT_2	-	Antitoxin
-	3337426..3337576	+	151	NuclAT_1	-	-
CDIF630erm_RS20760	3337498..3337641	-	144	WP_011861731.1	hypothetical protein	Toxin
CDIF630erm_RS15720	3337899..3338087	+	189	WP_009898401.1	hypothetical protein	-
CDIF630erm_RS15725	3338334..3339038	-	705	WP_011861732.1	hypothetical protein	-
CDIF630erm_RS15730	3339040..3339471	-	432	WP_011861052.1	protein-export chaperone SecB	-
CDIF630erm_RS15735	3339489..3340085	-	597	WP_011861051.1	hypothetical protein	-
CDIF630erm_RS15740	3340598..3340894	-	297	WP_011861049.1	hypothetical protein	-
CDIF630erm_RS15745	3340911..3341726	-	816	WP_011861048.1	N-acetylmuramoyl-L-alanine amidase	-
CDIF630erm_RS15750	3341723..3341983	-	261	WP_011861047.1	phage holin family protein	-
CDIF630erm_RS15755	3342003..3342233	-	231	WP_011861046.1	hemolysin XhlA family protein	-
CDIF630erm_RS15760	3342268..3342450	-	183	WP_011861045.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5399.34 Da
• Isoelectric Point: 10.8277

>T64415 WP_011861731.1 NZ_CP016318:c3337641-3337498 [Clostridioides difficile]
MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNKKRH

Nucleotide

• Length: 144 bp

>T64415 NZ_CP016318:c3337641-3337498 [Clostridioides difficile]
ATGAGCGAATTTTTACTAGGAGTGTTAGCTAGTTTAACAGCTAGCTTTATTACATATATTATTTCCAGAAAAGTAAAAAG
CCACTCTGGCAGGAGTGACTTTGAGCTTGATGTAAAAATCAAGTTTAATAAAAAACGACATTAA

Antitoxin

Nucleotide

• Length: 147 bp

>AT64415 NZ_CP016318:3337426-3337572 [Clostridioides difficile]
GTAAATTCGTATCAAAAACAAAAAAAGAAACTACAATCTATTAGCCTAGAGTGAAGTTTCATTAACTAAATATTAATGTC
GTTTTTTATTAAACTTGATTTTTACATCAAGCTCAAAGTCACTCCTGCCAGAGTGGCTTTTTACTTT

Structures

Toxin

• AlphaFold DB: A0A069A3R1

Antitoxin