Detailed information of TA system TA064227

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2778616..2778825
• Replicon: chromosome
• Accession: NZ_CP016104
• Organism: Clostridioides difficile strain DSM 29629

Toxin (Protein)

• Gene name: CD2517.1
• Uniprot ID: Q182K5
• Locus tag: CDIF29629_RS13195
• Protein ID: WP_004454815.1
• Coordinates: 2778667..2778825 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	2778616..2778750 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF29629_RS13180	2773723..2775351	+	1629	WP_022617147.1	aspartate 4-decarboxylase	-
CDIF29629_RS13185	2775472..2776467	+	996	WP_003431031.1	asparaginase	-
CDIF29629_RS13190	2776652..2777035	-	384	WP_070116827.1	BlaI/MecI/CopY family transcriptional regulator	-
-	2778616..2778750	+	135	NuclAT_2	-	Antitoxin
CDIF29629_RS13195	2778667..2778825	-	159	WP_004454815.1	hypothetical protein	Toxin
CDIF29629_RS13200	2779183..2780553	-	1371	WP_070116829.1	cell wall-binding protein Cwp29	-
CDIF29629_RS13205	2780835..2780933	+	99	Protein_2473	Arm DNA-binding domain-containing protein	-
CDIF29629_RS13210	2780952..2781374	+	423	WP_003431042.1	hypothetical protein	-
CDIF29629_RS13215	2781692..2781877	+	186	WP_107615967.1	tyrosine-type recombinase/integrase	-
CDIF29629_RS13220	2782240..2782320	-	81	Protein_2476	VOC family protein	-
CDIF29629_RS13225	2782360..2783028	-	669	WP_070116831.1	VanZ family protein	-
CDIF29629_RS13230	2783127..2783510	-	384	WP_074048203.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5996.20 Da
• Isoelectric Point: 11.3102

>T64227 WP_004454815.1 NZ_CP016104:c2778825-2778667 [Clostridioides difficile]
MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK

Nucleotide

• Length: 159 bp

>T64227 NZ_CP016104:c2778825-2778667 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGCATACTAGCAAGTCTATCTGCTAGCTTAATAGTTTATATAGCTAGCAAACTATTTAGAAA
GCGTAAAAAACCACTCAAAGCGGCAACTAAGAGTGGTTGGGAATTTGATTTAAAAATCAGATTCCATAAAACTAAGTAA

Antitoxin

Nucleotide

• Length: 135 bp

>AT64227 NZ_CP016104:2778616-2778750 [Clostridioides difficile]
AAGAAGAGCTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT

Structures

Toxin

• AlphaFold DB: A0A125V849

Antitoxin