Detailed information of TA system TA064223

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1756644..1756858
• Replicon: chromosome
• Accession: NZ_CP016104
• Organism: Clostridioides difficile strain DSM 29629

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: CDIF29629_RS08440
• Protein ID: WP_003429855.1
• Coordinates: 1756644..1756805 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1756725..1756858 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF29629_RS08390	1751984..1752217	+	234	WP_070113070.1	hypothetical protein	-
CDIF29629_RS08395	1752227..1752628	+	402	WP_021363493.1	hypothetical protein	-
CDIF29629_RS08400	1752622..1752969	+	348	WP_070113072.1	hypothetical protein	-
CDIF29629_RS08405	1752969..1753394	+	426	WP_038812299.1	HK97 gp10 family phage protein	-
CDIF29629_RS08410	1753387..1753824	+	438	WP_003433544.1	hypothetical protein	-
CDIF29629_RS08415	1753817..1753993	+	177	WP_070112848.1	hypothetical protein	-
CDIF29629_RS08420	1753994..1755304	+	1311	WP_070116964.1	phage tail sheath family protein	-
CDIF29629_RS08425	1755321..1755791	+	471	WP_003429851.1	phage tail tube protein	-
CDIF29629_RS08430	1755863..1756303	+	441	WP_003429853.1	phage portal protein	-
CDIF29629_RS08440	1756644..1756805	+	162	WP_003429855.1	hypothetical protein	Toxin
-	1756725..1756858	-	134	NuclAT_0	-	Antitoxin
-	1756725..1756858	-	134	NuclAT_1	-	Antitoxin
-	1757203..1757251	-	49	NuclAT_5	-	-
-	1757203..1757251	-	49	NuclAT_7	-	-
-	1758358..1758463	-	106	NuclAT_4	-	-
-	1758358..1758463	-	106	NuclAT_6	-	-
CDIF29629_RS08445	1759679..1759849	+	171	WP_021361718.1	ribbon-helix-helix domain-containing protein	-
CDIF29629_RS08450	1759977..1760819	+	843	WP_074048061.1	hypothetical protein	-
CDIF29629_RS08455	1760872..1761030	+	159	WP_021401161.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1725462..1792009	66547

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T64223 WP_003429855.1 NZ_CP016104:1756644-1756805 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T64223 NZ_CP016104:1756644-1756805 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCATAAGTTCAAAT
AG

Antitoxin

Nucleotide

• Length: 134 bp

>AT64223 NZ_CP016104:c1756858-1756725 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin