Detailed information of TA system TA064218

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2822656..2822865
• Replicon: chromosome
• Accession: NZ_CP016102
• Organism: Clostridioides difficile strain DSM 29627

Toxin (Protein)

• Gene name: CD2517.1
• Uniprot ID: -
• Locus tag: CDIF29627_RS13430
• Protein ID: WP_003431040.1
• Coordinates: 2822707..2822865 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	2822656..2822790 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF29627_RS13400	2817859..2818059	+	201	WP_003431034.1	cold-shock protein	-
CDIF29627_RS13405	2818490..2819827	+	1338	WP_003431035.1	ATP-binding protein	-
CDIF29627_RS13410	2819870..2820451	+	582	WP_003431036.1	accessory gene regulator B family protein	-
CDIF29627_RS13415	2820469..2820630	+	162	WP_003431037.1	cyclic lactone autoinducer peptide	-
CDIF29627_RS13420	2820652..2821449	-	798	WP_003431038.1	N-acetylmuramoyl-L-alanine amidase	-
CDIF29627_RS13425	2821446..2821706	-	261	WP_003431039.1	phage holin family protein	-
-	2822656..2822790	+	135	NuclAT_2	-	Antitoxin
CDIF29627_RS13430	2822707..2822865	-	159	WP_003431040.1	hypothetical protein	Toxin
CDIF29627_RS13435	2823223..2824593	-	1371	WP_003431041.1	cell wall-binding protein Cwp29	-
CDIF29627_RS13440	2824875..2824973	+	99	Protein_2522	Arm DNA-binding domain-containing protein	-
CDIF29627_RS13445	2824992..2825414	+	423	WP_003431042.1	hypothetical protein	-
CDIF29627_RS13450	2825732..2825917	+	186	WP_107615967.1	tyrosine-type recombinase/integrase	-
CDIF29627_RS13455	2826280..2826360	-	81	Protein_2525	VOC family protein	-
CDIF29627_RS13460	2826400..2827068	-	669	WP_003431044.1	VanZ family protein	-
CDIF29627_RS13465	2827167..2827550	-	384	WP_071599195.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2816694..2825414	8720

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5982.18 Da
• Isoelectric Point: 11.3102

>T64218 WP_003431040.1 NZ_CP016102:c2822865-2822707 [Clostridioides difficile]
MDNFLQGILASLSASLIVYVASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK

Nucleotide

• Length: 159 bp

>T64218 NZ_CP016102:c2822865-2822707 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGCATACTAGCAAGTCTATCTGCTAGCTTAATAGTTTATGTAGCTAGCAAACTATTTAGAAA
GCGTAAAAAACCACTCAAAGCGGCAACTAAGAGTGGTTGGGAATTTGATTTAAAAATCAGATTCCATAAAACTAAGTAA

Antitoxin

Nucleotide

• Length: 135 bp

>AT64218 NZ_CP016102:2822656-2822790 [Clostridioides difficile]
AAGAAGAGCTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT

Structures

Antitoxin