Detailed information of TA system TA064214

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1768762..1768976
• Replicon: chromosome
• Accession: NZ_CP016102
• Organism: Clostridioides difficile strain DSM 29627

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: CDIF29627_RS08530
• Protein ID: WP_003429855.1
• Coordinates: 1768762..1768923 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1768843..1768976 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF29627_RS08480	1764102..1764335	+	234	WP_003433536.1	hypothetical protein	-
CDIF29627_RS08485	1764345..1764746	+	402	WP_009888836.1	hypothetical protein	-
CDIF29627_RS08490	1764740..1765087	+	348	WP_003433540.1	hypothetical protein	-
CDIF29627_RS08495	1765087..1765512	+	426	WP_003433542.1	HK97 gp10 family phage protein	-
CDIF29627_RS08500	1765505..1765942	+	438	WP_003433544.1	hypothetical protein	-
CDIF29627_RS08505	1765935..1766111	+	177	WP_003433548.1	hypothetical protein	-
CDIF29627_RS08510	1766112..1767422	+	1311	WP_003433550.1	phage tail sheath family protein	-
CDIF29627_RS08515	1767439..1767909	+	471	WP_003429851.1	phage tail tube protein	-
CDIF29627_RS08520	1767981..1768421	+	441	WP_003429853.1	phage portal protein	-
CDIF29627_RS08530	1768762..1768923	+	162	WP_003429855.1	hypothetical protein	Toxin
-	1768843..1768976	-	134	NuclAT_0	-	Antitoxin
-	1768843..1768976	-	134	NuclAT_1	-	Antitoxin
-	1769321..1769369	-	49	NuclAT_5	-	-
-	1769321..1769369	-	49	NuclAT_6	-	-
CDIF29627_RS08535	1770248..1770823	+	576	WP_043897361.1	SHOCT domain-containing protein	-
CDIF29627_RS08540	1771127..1771300	+	174	WP_003433557.1	hypothetical protein	-
CDIF29627_RS19685	1771424..1772251	+	828	WP_003433559.1	hypothetical protein	-
CDIF29627_RS19690	1772300..1772476	+	177	WP_003433561.1	hypothetical protein	-
CDIF29627_RS08550	1772907..1773467	+	561	WP_021387709.1	ribosomal protein L7/L12	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1736544..1803165	66621

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T64214 WP_003429855.1 NZ_CP016102:1768762-1768923 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T64214 NZ_CP016102:1768762-1768923 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCATAAGTTCAAAT
AG

Antitoxin

Nucleotide

• Length: 134 bp

>AT64214 NZ_CP016102:c1768976-1768843 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin