Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2233981..2234507 Replicon chromosome
Accession NZ_CP016018
Organism Escherichia coli strain ER1821R

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag A9X67_RS11045 Protein ID WP_000323025.1
Coordinates 2234220..2234507 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag A9X67_RS11040 Protein ID WP_000534858.1
Coordinates 2233981..2234220 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9X67_RS23510 2229008..2229775 - 768 Protein_2097 exonuclease -
A9X67_RS10990 2229868..2230059 - 192 WP_001083297.1 lysis protein YdfD -
A9X67_RS10995 2230056..2230244 - 189 WP_000854559.1 cell division inhibition protein DicB -
A9X67_RS11005 2230812..2231030 - 219 WP_001171942.1 hypothetical protein -
A9X67_RS11010 2231190..2231345 - 156 WP_000379575.1 DUF1391 family protein -
A9X67_RS11015 2231512..2231919 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
A9X67_RS11020 2232003..2232233 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
A9X67_RS11025 2232217..2232495 + 279 Protein_2104 hypothetical protein -
A9X67_RS11030 2232530..2232679 + 150 WP_011443592.1 hypothetical protein -
A9X67_RS11035 2233116..2233448 - 333 WP_001301033.1 protein FlxA -
A9X67_RS23525 2233651..2233956 - 306 WP_001326990.1 hypothetical protein -
A9X67_RS11040 2233981..2234220 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
A9X67_RS11045 2234220..2234507 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
A9X67_RS11050 2234579..2234734 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
A9X67_RS11055 2234951..2235202 + 252 WP_000980994.1 hypothetical protein -
A9X67_RS11060 2235269..2235547 + 279 WP_012304870.1 hypothetical protein -
A9X67_RS11065 2235549..2236598 + 1050 WP_001265198.1 DUF968 domain-containing protein -
A9X67_RS11070 2236612..2237364 + 753 WP_001047135.1 antitermination protein -
A9X67_RS25285 2237642..2237731 - 90 WP_120795389.1 hypothetical protein -
A9X67_RS11075 2237786..2237998 - 213 WP_000087756.1 cold shock-like protein CspF -
A9X67_RS11080 2238299..2238514 + 216 WP_000066484.1 cold shock-like protein CspB -
A9X67_RS25780 2238878..2239048 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
A9X67_RS11085 2239268..2239483 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2227145..2248851 21706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T64139 WP_000323025.1 NZ_CP016018:2234220-2234507 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T64139 NZ_CP016018:2234220-2234507 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT64139 WP_000534858.1 NZ_CP016018:2233981-2234220 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT64139 NZ_CP016018:2233981-2234220 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References