Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | Zor-Orz/- |
| Location | 2953370..2953778 | Replicon | chromosome |
| Accession | NC_002655 | ||
| Organism | Escherichia coli O157:H7 str. EDL933 | ||
| T1TAdb ID | TA04739 | ||
Toxin (Protein)
| Gene name | ZorO | Uniprot ID | - |
| Locus tag | Z3289 | Protein ID | AAG57182.1 |
| Coordinates | 2953370..2953459 (-) | Length | 30 a.a. |
Antitoxin (RNA)
| Gene name | OrzO | ||
| Locus tag | orzO | ||
| Coordinates | 2953698..2953778 (+) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Z_RS15440 (Z3287) | 2949252..2952881 | + | 3630 | WP_000356841.1 | DUF4132 domain-containing protein | - |
| Z_RS15445 (Z3288) | 2952943..2953260 | + | 318 | WP_000636932.1 | hypothetical protein | - |
| - | 2953370..2953459 | - | 90 | - | - | Toxin |
| - | 2953698..2953778 | + | 81 | - | - | Antitoxin |
| Z_RS15460 (Z3291) | 2954501..2955589 | + | 1089 | WP_001302026.1 | MoxR family ATPase | - |
| Z_RS15465 (Z3292) | 2955600..2957129 | + | 1530 | WP_001294381.1 | hypothetical protein | - |
| Z_RS15470 (Z3293) | 2957148..2957879 | + | 732 | WP_000528951.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 30 a.a. Molecular weight: 3263.11 Da Isoelectric Point: 6.0104
>T6361 AAG57182.1 NC_002655:c2953459-2953370 [Escherichia coli O157:H7 str. EDL933]
MDTLTQKLTVLIAVLELLVALLRLIDLLK
MDTLTQKLTVLIAVLELLVALLRLIDLLK
Download Length: 90 bp
>T6361 NC_002655:c2953459-2953370 [Escherichia coli O157:H7 str. EDL933]
ATGGACACGCTGACACAAAAGTTAACCGTGCTCATTGCCGTACTGGAGTTATTAGTGGCTCTGTTACGGTTGATTGATTT
GTTGAAGTAA
ATGGACACGCTGACACAAAAGTTAACCGTGCTCATTGCCGTACTGGAGTTATTAGTGGCTCTGTTACGGTTGATTGATTT
GTTGAAGTAA
Antitoxin
Download Length: 81 bp
>AT6361 NC_002655:2953698-2953778 [Escherichia coli O157:H7 str. EDL933]
GTTGGTACGAAATGTTGCACAATCAGCAAGTTAGCGTCACTGTAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTT
T
GTTGGTACGAAATGTTGCACAATCAGCAAGTTAGCGTCACTGTAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTT
T
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10213 | Escherichia coli O157:H7 str. EDL933 |
96.552 |
100 |
0.966 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Jia Wen et al. (2014) The ZorO-OrzO type I toxin-antitoxin locus: repression by the OrzO antitoxin. Nucleic Acids Research 42(3):1930-46. [PubMed:24203704]
(2) Bikash Bogati et al. (2021) Improved growth of Escherichia coli in aminoglycoside antibiotics by the zor-orz toxin-antitoxin system. Journal of Bacteriology 204(1):JB0040721. [PubMed:34570627]