Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | fst-RNAII/- |
| Location | 4038..4281 | Replicon | plasmid pAD1 |
| Accession | NG_034485 | ||
| Organism | Enterococcus faecalis | ||
| T1TAdb ID | TA04741 | ||
Toxin (Protein)
| Gene name | fst | Uniprot ID | - |
| Locus tag | Fst | Protein ID | YP_009074128.1 |
| Coordinates | 4180..4281 (-) | Length | 34 a.a. |
Antitoxin (RNA)
| Gene name | RNAII | ||
| Locus tag | RNAII | ||
| Coordinates | 4076..4140 (+) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Locus_0 | 155..1165 | - | 1011 | YP_009074123.1 | replication-associated protein | - |
| Locus_1 | 1606..2451 | + | 846 | YP_009074124.1 | replication-associated protein | - |
| Locus_2 | 2444..2815 | + | 372 | YP_009074125.1 | replication-associated protein | - |
| Locus_3 | 2919..3209 | - | 291 | YP_009074126.1 | unknown | - |
| Locus_4 | 3380..3841 | - | 462 | YP_009074127.1 | unknown | - |
| - | 4076..4140 | + | 65 | - | - | Antitoxin |
| Locus_5 | 4180..4281 | - | 102 | YP_009074128.1 | unknown | Toxin |
| Locus_6 | 4372..4584 | - | 213 | YP_009074129.1 | unknown | - |
| Locus_7 | 4541..4891 | - | 351 | YP_009074130.1 | unknown | - |
| Locus_8 | 4888..5145 | - | 258 | YP_009074131.1 | unknown | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..5145 | 5145 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 34 a.a. Molecular weight: 3731.42 Da Isoelectric Point: 4.1672
>T6359 YP_009074128.1 NG_034485:c4281-4180 [Enterococcus faecalis]
VKDLMSLVIAPIFVGLVLEMISRVLDEEDDSRK
VKDLMSLVIAPIFVGLVLEMISRVLDEEDDSRK
Download Length: 102 bp
>T6359 NG_034485:c4281-4180 [Enterococcus faecalis]
GTGAAAGATTTAATGTCGTTGGTTATCGCACCAATCTTTGTAGGATTGGTTCTGGAAATGATTTCTCGTGTGTTGGACGA
GGAAGACGATAGCCGAAAGTAA
GTGAAAGATTTAATGTCGTTGGTTATCGCACCAATCTTTGTAGGATTGGTTCTGGAAATGATTTCTCGTGTGTTGGACGA
GGAAGACGATAGCCGAAAGTAA
Antitoxin
Download Length: 65 bp
>AT6359 NG_034485:4076-4140 [Enterococcus faecalis]
AACGACATTAAATCGTACAGATAACACAAAAAGCAATCCTACGGCGAATAGGATTGCTTTTTTTT
AACGACATTAAATCGTACAGATAACACAAAAAGCAATCCTACGGCGAATAGGATTGCTTTTTTTT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10028 | Enterococcus faecalis V583 |
60.87 |
100 |
0.609 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) T J Greenfield et al. (2001) Antisense RNA regulation of the par post-segregational killing system: structural analysis and mechanism of binding of the antisense RNA, RNAII and its target, RNAI. Molecular Microbiology 42(2):527-37. [PubMed:11703673]