6319

Detailed information of TA system    

experimentalExperimentally validated    

Overview


TA module


Type IV Classification (family/domain) abiEi-abiEii/-
Location 1296512..1297944 Replicon chromosome
Accession NC_004116
Organism Streptococcus agalactiae 2603V/R

Toxin (Protein)


Gene name abiEi Uniprot ID Q8DZ35
Locus tag SAG1285 Protein ID WP_001030302.1
Coordinates 1297099..1297944 (+) Length 282 a.a.

Antitoxin (Protein)


Gene name abiEii Uniprot ID Q8DZ36
Locus tag SAG1284 Protein ID WP_000037239.1
Coordinates 1296512..1297102 (+) Length 197 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SAG_RS09115 (SAG1284) 1296512..1297102 + 591 WP_000037239.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
SAG_RS09120 (SAG1285) 1297099..1297944 + 846 WP_001030302.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
SAG_RS09125 (SAG1286) 1298030..1300831 - 2802 WP_000656702.1 CHAP domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 282 a.a.        Molecular weight: 32414.90 Da        Isoelectric Point: 5.2111

>T6319 WP_001030302.1 NC_004116:1297099-1297944 [Streptococcus agalactiae 2603V/R]
MNKAKLTALCHKISKNTGLTFNSVMTYYFLEVILKKLSQSSYSNHYIFKGGFLLSNVIGVESRSTVDIDFLFHQITLSEE
TVKQQLKEILADSEEGISFVIQSITTIKESDDYGGYRATISCQLENIKQVIHLDIATGDVVTPQPITYDYKAIFDEDNFP
IIAYTIETILAEKLQTIYSRNFLNSRSKDFYDVYILSKLKKKDIDFNQLKNACQRTFSYRETELDFEKIIELLERFKSDP
TQNQQWQNYSKKYSYTKGISLANVLDEMISLITIIISTDSD

Download         Length: 846 bp

>T6319 NC_004116:1297099-1297944 [Streptococcus agalactiae 2603V/R]
ATGAACAAAGCTAAATTAACAGCACTCTGTCATAAAATATCAAAGAATACTGGTTTAACCTTTAATTCCGTGATGACCTA
TTACTTCCTAGAAGTCATTTTGAAAAAATTAAGTCAAAGTTCTTATTCAAATCACTATATCTTCAAAGGAGGATTCCTAC
TGTCGAATGTCATCGGAGTTGAATCTCGTAGCACTGTTGATATTGACTTTTTATTCCATCAAATAACACTCTCAGAAGAA
ACTGTTAAACAGCAACTCAAGGAAATTTTAGCAGATTCCGAAGAAGGTATATCTTTTGTGATTCAATCAATCACAACTAT
TAAAGAAAGTGATGACTATGGTGGCTATCGAGCAACAATTTCGTGCCAACTTGAGAATATTAAACAAGTTATCCATTTGG
ATATTGCGACAGGTGATGTTGTAACTCCTCAGCCGATAACATACGACTATAAAGCTATTTTTGATGAAGACAATTTTCCA
ATCATTGCTTATACAATTGAAACAATTCTAGCTGAAAAACTCCAAACCATCTATTCACGAAACTTCTTGAACAGCAGAAG
CAAAGACTTTTACGATGTTTATATTCTTTCAAAATTGAAGAAAAAAGATATTGATTTCAATCAGTTAAAAAATGCTTGCC
AAAGAACTTTTTCCTATCGCGAAACTGAGCTCGATTTCGAAAAAATTATTGAACTACTCGAGAGATTCAAATCTGATCCT
ACTCAGAATCAACAATGGCAAAATTATTCAAAAAAATATAGTTATACAAAAGGAATTTCACTTGCAAATGTGCTTGATGA
GATGATTAGTCTTATTACAATTATTATTTCTACTGACTCTGATTAA

Antitoxin


Download         Length: 197 a.a.        Molecular weight: 23074.63 Da        Isoelectric Point: 7.8056

>AT6319 WP_000037239.1 NC_004116:1296512-1297102 [Streptococcus agalactiae 2603V/R]
MSKKEILLDFIEKNNGIVTNKDCKALGIPTIYLTRLEKEGIIFRVEKGIFLTQNGDYDEYYFFQYRFPKAIFSYISALYL
QQFTDEIPQYFDVTVPRGYRFNTPPANLNIHFVSKEYSELGMTTVPTPMGNNVRVYDFERIICDFVIHREKIDSELFVKT
LQSYGNYPKKNLAKLYEYATKMNTLEKVKQTLEVLI

Download         Length: 591 bp

>AT6319 NC_004116:1296512-1297102 [Streptococcus agalactiae 2603V/R]
ATGTCAAAAAAAGAGATTCTACTCGATTTTATAGAAAAGAACAATGGCATTGTCACAAACAAAGATTGTAAAGCACTAGG
TATTCCAACAATATATCTGACAAGACTAGAGAAAGAAGGCATTATCTTCCGAGTTGAAAAAGGTATTTTCCTAACCCAAA
ACGGAGACTATGATGAATACTACTTCTTTCAATATCGTTTTCCTAAGGCTATTTTCTCTTATATTTCGGCACTGTATCTA
CAACAGTTTACAGATGAAATTCCACAATATTTTGATGTGACAGTTCCTAGAGGCTACCGCTTTAATACTCCTCCAGCTAA
TCTAAACATTCACTTTGTCTCTAAAGAATACAGCGAGCTGGGAATGACTACTGTACCTACTCCTATGGGAAACAACGTAA
GGGTATATGATTTTGAACGTATTATTTGTGATTTTGTAATACATCGAGAAAAGATTGATTCCGAACTCTTTGTCAAGACA
CTTCAATCTTATGGGAACTATCCTAAAAAAAATCTAGCAAAACTCTATGAATATGCGACAAAAATGAATACTTTAGAAAA
AGTTAAACAAACTCTGGAGGTTCTAATATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T10047 Mycoplasma mycoides subsp. capri str. GM12

37.729

98.556

0.372


Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT10047 Mycoplasma mycoides subsp. capri str. GM12

42.929

100

0.434


Multiple sequence alignment    




Structures


Toxin

Source ID Structure
AlphaFold DB A8D822


Antitoxin

Source ID Structure
PDB 6Y8Q
AlphaFold DB A0A2I7ZET8

References


(1) Ron L Dy et al. (2014) A widespread bacteriophage abortive infection system functions through a Type IV toxin-antitoxin mechanism. Nucleic Acids Research 42(7):4590-605. [PubMed:24465005] experimental

(2) Hannah G Hampton et al. (2018) AbiEi Binds Cooperatively to the Type IV abiE Toxin-Antitoxin Operator Via a Positively-Charged Surface and Causes DNA Bending and Negative Autoregulation. Journal of Molecular Biology 430(8):1141-1156. [PubMed:29518409] experimental


experimental experimental literature