10047

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type IV Classification (family/domain) abiEi-abiEii/-
Location 210859..212294 Replicon chromosome
Accession NZ_CP001668
Organism Mycoplasma mycoides subsp. capri str. GM12

Toxin (Protein)


Gene name abiEii Uniprot ID -
Locus tag MMCAP2_RS00810 Protein ID Protein_154
Coordinates 210859..211698 (-) Length 280 a.a.

Antitoxin (Protein)


Gene name abiEi Uniprot ID A0A7Z7LCJ7
Locus tag MMCAP2_RS00815 Protein ID WP_020862546.1
Coordinates 211698..212294 (-) Length 199 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MMCAP2_RS00795 (MMCAP2_0156) 205960..206625 - 666 WP_020862542.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
MMCAP2_RS00800 (MMCAP2_0157) 206627..208030 - 1404 WP_020862543.1 magnesium transporter -
MMCAP2_RS05055 (MMCAP2_0159) 208710..210839 + 2130 WP_020862544.1 lipoprotein -
MMCAP2_RS00810 (MMCAP2_0160) 210859..211698 - 840 Protein_154 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
MMCAP2_RS00815 (MMCAP2_0161) 211698..212294 - 597 WP_020862546.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
MMCAP2_RS00820 (MMCAP2_0162) 212505..213711 - 1207 Protein_156 BspA family leucine-rich repeat surface protein -
MMCAP2_RS00825 (MMCAP2_0163) 213825..216515 - 2691 WP_020862547.1 alanine--tRNA ligase -
MMCAP2_RS00830 (MMCAP2_0164) 216589..217083 - 495 WP_017698022.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(32-262)

Antitoxin

Transcriptional regulator, AbiEi antitoxin

(6-53)


Sequences

Toxin        


Download         Length: 280 a.a.        Molecular weight: 32819.74 Da        Isoelectric Point: 9.4031

>T10047 Protein_154 NZ_CP001668:c211698-210859 [Mycoplasma mycoides subsp. capri str. GM12]
MNLQKLKAICKRLSNTKTNYNIILKHYFMISILEKIVKSKNKQNFIIKGGFLLSNMMSLNERTTQDIDCLIKGINFVKEN
ILKIISDVLRYDFTDFIEYEIKEITEIKKRDKYNGFRVKILCKLEKLEEIVKIDLAIGDVITPSEIKYNFKNIFSNSSFE
IQAYNLETILAEKIFIINNLNIQSTRIKDLYDIFLIYSLKEFEIDYNILKCACLNTFKKRNTVFNIDLILNLLNEINQLD
IFKLLNNYKQKYFYAQKIEFEILVESVIKLLEKLKSS

Download         Length: 840 bp

>T10047 NZ_CP001668:c211698-210859 [Mycoplasma mycoides subsp. capri str. GM12]
ATGAACTTACAAAAATTAAAAGCAATTTGTAAAAGGTTATCAAATTAAACAAAAACAAATTATAATATCATCTTAAAACA
TTACTTTATGATATCCATATTAGAAAAAATTGTTAAAAGTAAAAATAAGCAAAACTTTATAATTAAAGGCGGATTCTTAC
TTTCTAATATGATGAGTTTAAACGAAAGAACCACTCAAGATATAGATTGCCTAATAAAGGGTATCAATTTTGTTAAAGAG
AATATTCTAAAAATTATTTCTGATGTACTAAGATATGATTTTACTGACTTTATAGAATATGAAATAAAAGAAATAACAGA
AATAAAAAAGAGAGACAAATATAATGGATTTAGAGTCAAAATATTATGTAAATTAGAAAAGTTAGAAGAAATAGTAAAAA
TAGATTTAGCAATTGGTGATGTTATAACACCATCTGAGATTAAATACAATTTTAAAAATATCTTTTCAAATAGTAGCTTT
GAAATACAAGCATATAATTTAGAAACGATATTAGCTGAAAAAATATTTATAATTAATAATTTAAATATACAATCAACTAG
AATAAAAGATCTTTATGATATTTTTTTAATATATAGTTTGAAAGAGTTTGAGATTGATTATAATATTCTAAAATGTGCTT
GTTTAAATACTTTTAAAAAAAGAAATACTGTTTTTAATATAGATTTAATACTTAATTTATTAAATGAAATTAACCAATTA
GATATTTTTAAATTACTTTGAAATAATTACAAGCAAAAATACTTTTACGCTCAAAAAATAGAATTTGAAATACTGGTGGA
ATCTGTAATTAAGCTTTTAGAAAAGTTAAAAAGCTCTTAG

Antitoxin


Download         Length: 199 a.a.        Molecular weight: 23622.23 Da        Isoelectric Point: 9.3421

>AT10047 WP_020862546.1 NZ_CP001668:c212294-211698 [Mycoplasma mycoides subsp. capri str. GM12]
MNYKEQLIKEAKKRDGLITRKEIINLKIPSIYITRMIRSNELEKVDIGIYALTSENWHYDPDYNFSIRHKTPIFSYTYAL
TFFDFTDVIPMFRDVTVYHGYNAHNLDPQTRVHYVSKDIYELGVVEVKTNCGNKVRAYNIERTICDFIKHRDKIEAELFA
KTMYKYSEYEKRDKKLHEYAKKMNIGKKVYEVFQVLV

Download         Length: 597 bp

>AT10047 NZ_CP001668:c212294-211698 [Mycoplasma mycoides subsp. capri str. GM12]
ATGAATTATAAAGAACAACTGATAAAAGAAGCCAAAAAAAGAGACGGTCTTATTACAAGAAAAGAAATTATAAATTTAAA
AATTCCATCAATTTATATTACAAGAATGATTAGAAGTAATGAATTAGAAAAAGTCGATATTGGTATTTATGCTCTAACTT
CTGAAAATTGGCACTATGATCCTGATTATAATTTTAGTATCAGACATAAAACACCTATTTTTTCTTATACTTATGCACTT
ACATTTTTTGATTTTACAGATGTTATTCCAATGTTTCGTGATGTAACTGTTTATCATGGATATAATGCTCATAATTTAGA
TCCACAAACTAGAGTACACTATGTTTCAAAAGATATATATGAACTTGGAGTTGTAGAAGTAAAAACAAATTGTGGTAATA
AAGTTAGAGCTTATAATATTGAAAGAACAATTTGTGACTTTATTAAACATAGAGATAAAATCGAAGCTGAACTATTTGCA
AAAACTATGTATAAATATTCTGAATATGAAAAAAGAGATTGAAAAAAACTTCATGAATATGCTAAAAAAATGAACATTGG
TAAGAAAGTTTATGAAGTTTTTCAAGTATTAGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6319 Streptococcus agalactiae 2603V/R

37.729

98.556

0.372

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT6319 Streptococcus agalactiae 2603V/R

42.929

100

0.434

Multiple sequence alignment    




Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A7Z7LCJ7

References

(1) Virginia Hill et al. (2021) Minimalistic mycoplasmas harbor different functional toxin-antitoxin systems. PLoS Genetics 17(10):e1009365. [PubMed:34673769] experimental


experimental experimental literature