Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1749515..1750041 Replicon chromosome
Accession NZ_CP014272
Organism Escherichia coli K-12 strain K-12 C3026

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag C3026_RS08995 Protein ID WP_000323025.1
Coordinates 1749515..1749802 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag C3026_RS09000 Protein ID WP_000534858.1
Coordinates 1749802..1750041 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3026_RS08955 1744539..1744754 - 216 WP_000839590.1 phage lysis protein EssD -
C3026_RS28690 1744974..1745144 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
C3026_RS08960 1745508..1745723 - 216 WP_000066484.1 cold shock-like protein CspB -
C3026_RS08965 1746024..1746236 + 213 WP_000087756.1 cold shock-like protein CspF -
C3026_RS28105 1746291..1746380 + 90 WP_120795389.1 hypothetical protein -
C3026_RS08970 1746658..1747410 - 753 WP_001047135.1 antitermination protein -
C3026_RS08975 1747424..1748473 - 1050 WP_001265198.1 DUF968 domain-containing protein -
C3026_RS08980 1748475..1748753 - 279 WP_012304870.1 hypothetical protein -
C3026_RS08985 1748820..1749071 - 252 WP_000980994.1 hypothetical protein -
C3026_RS08990 1749288..1749443 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
C3026_RS08995 1749515..1749802 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
C3026_RS09000 1749802..1750041 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
C3026_RS25810 1750066..1750371 + 306 WP_001326990.1 hypothetical protein -
C3026_RS09005 1750574..1750906 + 333 WP_001301033.1 protein FlxA -
C3026_RS09010 1751343..1751492 - 150 WP_011443592.1 hypothetical protein -
C3026_RS09015 1751527..1751805 - 279 Protein_1754 hypothetical protein -
C3026_RS09020 1751789..1752019 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
C3026_RS09025 1752103..1752510 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
C3026_RS09030 1752677..1752832 + 156 WP_000379575.1 DUF1391 family protein -
C3026_RS09035 1752992..1753210 + 219 WP_001171942.1 hypothetical protein -
C3026_RS09045 1753778..1753966 + 189 WP_000854559.1 cell division inhibition protein DicB -
C3026_RS09050 1753963..1754154 + 192 WP_001083297.1 lysis protein YdfD -
C3026_RS25825 1754247..1755014 + 768 Protein_1761 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1735171..1759420 24249


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T60579 WP_000323025.1 NZ_CP014272:c1749802-1749515 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T60579 NZ_CP014272:c1749802-1749515 [Escherichia coli K-12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT60579 WP_000534858.1 NZ_CP014272:c1750041-1749802 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT60579 NZ_CP014272:c1750041-1749802 [Escherichia coli K-12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References