60527

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1550451..1550977 Replicon chromosome
Accession NZ_CP014270
Organism Escherichia coli K-12 strain K-12 DHB4

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag DHB4_RS07765 Protein ID WP_000323025.1
Coordinates 1550451..1550738 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag DHB4_RS07770 Protein ID WP_000534858.1
Coordinates 1550738..1550977 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DHB4_RS07725 1545475..1545690 - 216 WP_000839590.1 phage lysis protein EssD -
DHB4_RS27155 1545910..1546080 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
DHB4_RS07730 1546444..1546659 - 216 WP_000066484.1 cold shock-like protein CspB -
DHB4_RS07735 1546960..1547172 + 213 WP_000087756.1 cold shock-like protein CspF -
DHB4_RS26560 1547227..1547316 + 90 WP_120795389.1 hypothetical protein -
DHB4_RS07740 1547594..1548346 - 753 WP_001047135.1 antitermination protein -
DHB4_RS07745 1548360..1549409 - 1050 WP_001265198.1 DUF968 domain-containing protein -
DHB4_RS07750 1549411..1549689 - 279 WP_012304870.1 hypothetical protein -
DHB4_RS07755 1549756..1550007 - 252 WP_000980994.1 hypothetical protein -
DHB4_RS07760 1550224..1550379 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
DHB4_RS07765 1550451..1550738 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
DHB4_RS07770 1550738..1550977 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
DHB4_RS24470 1551002..1551307 + 306 WP_001326990.1 hypothetical protein -
DHB4_RS07775 1551510..1551842 + 333 WP_001301033.1 protein FlxA -
DHB4_RS07780 1552279..1552428 - 150 WP_011443592.1 hypothetical protein -
DHB4_RS07785 1552463..1552741 - 279 Protein_1491 hypothetical protein -
DHB4_RS07790 1552725..1552955 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
DHB4_RS07795 1553039..1553446 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
DHB4_RS07800 1553613..1553768 + 156 WP_000379575.1 DUF1391 family protein -
DHB4_RS07805 1553928..1554146 + 219 WP_001171942.1 hypothetical protein -
DHB4_RS07815 1554714..1554902 + 189 WP_000854559.1 cell division inhibition protein DicB -
DHB4_RS07820 1554899..1555090 + 192 WP_001083297.1 lysis protein YdfD -
DHB4_RS24485 1555183..1555950 + 768 Protein_1498 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1536107..1559149 23042


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T60527 WP_000323025.1 NZ_CP014270:c1550738-1550451 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T60527 NZ_CP014270:c1550738-1550451 [Escherichia coli K-12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT60527 WP_000534858.1 NZ_CP014270:c1550977-1550738 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT60527 NZ_CP014270:c1550977-1550738 [Escherichia coli K-12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References