Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1550451..1550977 Replicon chromosome
Accession NZ_CP014270
Organism Escherichia coli K-12 strain K-12 DHB4

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag DHB4_RS07765 Protein ID WP_000323025.1
Coordinates 1550451..1550738 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag DHB4_RS07770 Protein ID WP_000534858.1
Coordinates 1550738..1550977 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DHB4_RS07725 1545475..1545690 - 216 WP_000839590.1 phage lysis protein EssD -
DHB4_RS27155 1545910..1546080 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
DHB4_RS07730 1546444..1546659 - 216 WP_000066484.1 cold shock-like protein CspB -
DHB4_RS07735 1546960..1547172 + 213 WP_000087756.1 cold shock-like protein CspF -
DHB4_RS26560 1547227..1547316 + 90 WP_120795389.1 hypothetical protein -
DHB4_RS07740 1547594..1548346 - 753 WP_001047135.1 antitermination protein -
DHB4_RS07745 1548360..1549409 - 1050 WP_001265198.1 DUF968 domain-containing protein -
DHB4_RS07750 1549411..1549689 - 279 WP_012304870.1 hypothetical protein -
DHB4_RS07755 1549756..1550007 - 252 WP_000980994.1 hypothetical protein -
DHB4_RS07760 1550224..1550379 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
DHB4_RS07765 1550451..1550738 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
DHB4_RS07770 1550738..1550977 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
DHB4_RS24470 1551002..1551307 + 306 WP_001326990.1 hypothetical protein -
DHB4_RS07775 1551510..1551842 + 333 WP_001301033.1 protein FlxA -
DHB4_RS07780 1552279..1552428 - 150 WP_011443592.1 hypothetical protein -
DHB4_RS07785 1552463..1552741 - 279 Protein_1491 hypothetical protein -
DHB4_RS07790 1552725..1552955 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
DHB4_RS07795 1553039..1553446 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
DHB4_RS07800 1553613..1553768 + 156 WP_000379575.1 DUF1391 family protein -
DHB4_RS07805 1553928..1554146 + 219 WP_001171942.1 hypothetical protein -
DHB4_RS07815 1554714..1554902 + 189 WP_000854559.1 cell division inhibition protein DicB -
DHB4_RS07820 1554899..1555090 + 192 WP_001083297.1 lysis protein YdfD -
DHB4_RS24485 1555183..1555950 + 768 Protein_1498 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1536107..1559149 23042


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T60527 WP_000323025.1 NZ_CP014270:c1550738-1550451 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T60527 NZ_CP014270:c1550738-1550451 [Escherichia coli K-12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT60527 WP_000534858.1 NZ_CP014270:c1550977-1550738 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT60527 NZ_CP014270:c1550977-1550738 [Escherichia coli K-12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References