Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) symE-ohsC/SymE(toxin)
Location 2776588..2777000 Replicon chromosome
Accession NZ_CP014225
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name symE Uniprot ID U9YSY7
Locus tag AW869_RS13660 Protein ID WP_000132601.1
Coordinates 2776588..2776929 (-) Length 114 a.a.

Antitoxin (RNA)


Gene name ohsC
Locus tag -
Coordinates 2776924..2777000 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AW869_RS13650 2774001..2775047 - 1047 WP_000437621.1 5-methylcytosine-specific restriction endonuclease system specificity protein McrC -
AW869_RS13655 2775047..2776426 - 1380 WP_000443951.1 5-methylcytosine-specific restriction endonuclease subunit McrB -
AW869_RS13660 2776588..2776929 - 342 WP_000132601.1 endoribonuclease SymE Toxin
- 2776924..2777000 + 77 NuclAT_14 - Antitoxin
- 2776924..2777000 + 77 NuclAT_14 - Antitoxin
- 2776924..2777000 + 77 NuclAT_14 - Antitoxin
- 2776924..2777000 + 77 NuclAT_14 - Antitoxin
- 2776924..2777000 + 77 NuclAT_15 - Antitoxin
- 2776924..2777000 + 77 NuclAT_15 - Antitoxin
- 2776924..2777000 + 77 NuclAT_15 - Antitoxin
- 2776924..2777000 + 77 NuclAT_15 - Antitoxin
AW869_RS13665 2777157..2778551 - 1395 WP_001272447.1 type I restriction-modification system specificity subunit -
AW869_RS13670 2778548..2780137 - 1590 WP_001063204.1 type I restriction-modification system methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2769503..2778551 9048


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SymE-like

(20-71)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12203.02 Da        Isoelectric Point: 8.5012

>T60394 WP_000132601.1 NZ_CP014225:c2776929-2776588 [Escherichia coli str. K-12 substr. MG1655]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Download         Length: 342 bp

>T60394 NZ_CP014225:c2776929-2776588 [Escherichia coli str. K-12 substr. MG1655]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin


Download         Length: 77 bp

>AT60394 NZ_CP014225:2776924-2777000 [Escherichia coli str. K-12 substr. MG1655]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YSY7

Antitoxin

Download structure file

References