Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yxiD-yxxD/- |
| Location | 4036344..4038493 | Replicon | chromosome |
| Accession | NC_000964 | ||
| Organism | Bacillus subtilis subsp. subtilis str. 168 | ||
Toxin (Protein)
| Gene name | yxiD | Uniprot ID | P42296 |
| Locus tag | BSU_39300 | Protein ID | NP_391809.2 |
| Coordinates | 4036784..4038493 (-) | Length | 570 a.a. |
Antitoxin (Protein)
| Gene name | yxxD | Uniprot ID | P40737 |
| Locus tag | BSU_39290 | Protein ID | NP_391808.1 |
| Coordinates | 4036344..4036787 (-) | Length | 148 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BSU_39250 (BSU39250) | 4031797..4032243 | - | 447 | NP_391804.1 | phosphate starvation protein (universal stress protein A family) | - |
| BSU_39260 (BSU39260) | 4032346..4033755 | - | 1410 | NP_391805.2 | aryl-phospho-beta-d-glucosidase | - |
| BSU_39270 (BSU39270) | 4033778..4035607 | - | 1830 | NP_391806.2 | phosphotransferase system (PTS) beta-glucoside-specific enzyme IIBCA component | - |
| BSU_39280 (BSU39280) | 4035990..4036298 | - | 309 | NP_391807.1 | hypothetical protein | - |
| BSU_39290 (BSU39290) | 4036344..4036787 | - | 444 | NP_391808.1 | antitoxin factor of the RttD-RttE toxin-antitoxin system | Antitoxin |
| BSU_39300 (BSU39300) | 4036784..4038493 | - | 1710 | NP_391809.2 | ribonuclease toxin of the RtbD-RtbE toxin-antitoxin system | Toxin |
| BSU_39310 (BSU39310) | 4038513..4038782 | - | 270 | NP_391810.1 | hypothetical protein | - |
| BSU_39320 (BSU39320) | 4038794..4039159 | - | 366 | NP_391811.1 | hypothetical protein | - |
| BSU_39330 (BSU39330) | 4039466..4040875 | - | 1410 | NP_391812.2 | arabinan endo-1,5-alpha-L-arabinosidase ([Ca(2+)-dependent] | - |
| BSU_39339 (BSU39339) | 4041009..4041173 | - | 165 | YP_003097797.1 | hypothetical protein | - |
| BSU_39340 (BSU39340) | 4041492..4041938 | + | 447 | NP_391813.2 | transcriptional antiterminator | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 570 a.a. Molecular weight: 64326.29 Da Isoelectric Point: 9.5906
>T6030 NP_391809.2 NC_000964:c4038493-4036784 [Bacillus subtilis subsp. subtilis str. 168]
MKTLDVHALHEGIQHTIEKLDKQKQQLEKLEKSVEHLAGMKDALKGKGGDAIRTFYEECHKPFLLFFGIFIDEYKKVLKQ
TQHAISSVESNSHGMIAEAFLSHDARHGVKHAREVTEQLTDAVNRQTSAIDHIVSLPTVNDSFFRMETEQAERLISDTLN
KLFQFDGQQTQALEAAKSDFQTMKKYIDQLETMYTGPKIEITGYKSGSILKSQEEENINQIFGAINPQMKQADDSPMEMM
LKKLAENEKSKVDSVVKTGDSKKVSKNIIVINGKVYNTSEHREHIKTDFSNAEVKQVVYNDTLYNVYISGNDMKLEPVVS
LSDIKVDENGYVKILETAVELTGVYDLFKAATGRDPVSGEKVTGKDRVVASINSVPFAKIAKLEKLIDINKLINNGKKAK
KASEVKNVAKDKGKIANDVSGSANKINSDLIKKYARDIEQRTGRELPKNQIDKLKEALRNKEYKKMSPIETAKHRTKFDK
VKNKVIKEWEENTGQKWPVYKENVVSEKTGKIIRKKGDKYDAHHIIENTFGGEHEWWNMHPAKFPNEHQAGIHGTGSPAN
ELFKGGKKK
MKTLDVHALHEGIQHTIEKLDKQKQQLEKLEKSVEHLAGMKDALKGKGGDAIRTFYEECHKPFLLFFGIFIDEYKKVLKQ
TQHAISSVESNSHGMIAEAFLSHDARHGVKHAREVTEQLTDAVNRQTSAIDHIVSLPTVNDSFFRMETEQAERLISDTLN
KLFQFDGQQTQALEAAKSDFQTMKKYIDQLETMYTGPKIEITGYKSGSILKSQEEENINQIFGAINPQMKQADDSPMEMM
LKKLAENEKSKVDSVVKTGDSKKVSKNIIVINGKVYNTSEHREHIKTDFSNAEVKQVVYNDTLYNVYISGNDMKLEPVVS
LSDIKVDENGYVKILETAVELTGVYDLFKAATGRDPVSGEKVTGKDRVVASINSVPFAKIAKLEKLIDINKLINNGKKAK
KASEVKNVAKDKGKIANDVSGSANKINSDLIKKYARDIEQRTGRELPKNQIDKLKEALRNKEYKKMSPIETAKHRTKFDK
VKNKVIKEWEENTGQKWPVYKENVVSEKTGKIIRKKGDKYDAHHIIENTFGGEHEWWNMHPAKFPNEHQAGIHGTGSPAN
ELFKGGKKK
Download Length: 1710 bp
>T6030 NC_000964:c4038493-4036784 [Bacillus subtilis subsp. subtilis str. 168]
TTGAAAACATTAGATGTCCACGCTCTTCACGAGGGCATTCAGCATACGATTGAGAAACTGGACAAGCAAAAACAACAGCT
GGAGAAGCTGGAAAAAAGCGTTGAGCATCTGGCAGGCATGAAGGATGCGCTGAAAGGAAAAGGCGGAGATGCCATCCGGA
CCTTTTACGAGGAGTGCCACAAGCCTTTTCTCCTTTTCTTTGGCATATTTATCGACGAATACAAAAAAGTGCTGAAACAA
ACACAGCATGCGATTTCCTCTGTAGAATCTAACTCCCACGGCATGATCGCAGAGGCTTTTCTCTCACACGATGCGAGACA
TGGGGTCAAGCATGCACGAGAAGTGACAGAGCAGCTCACTGACGCAGTCAATCGCCAGACATCAGCCATAGATCACATCG
TCAGCCTGCCGACAGTCAATGATTCCTTTTTTCGGATGGAAACAGAACAGGCTGAGCGGCTGATTTCAGACACCCTGAAT
AAGCTCTTTCAATTTGACGGCCAGCAAACCCAAGCACTAGAAGCCGCAAAATCAGATTTTCAAACGATGAAAAAATACAT
TGATCAGCTCGAAACCATGTACACCGGCCCGAAAATTGAAATCACAGGCTACAAAAGCGGCTCCATCCTGAAATCACAGG
AAGAAGAAAACATCAACCAAATCTTCGGCGCGATTAACCCGCAAATGAAGCAGGCGGATGATTCACCGATGGAGATGATG
CTGAAGAAATTAGCCGAGAATGAAAAGTCTAAAGTGGATTCAGTTGTCAAAACGGGTGATTCTAAAAAGGTAAGTAAAAA
TATCATCGTTATCAATGGTAAAGTGTATAATACGAGTGAACATAGAGAACATATAAAAACTGATTTCTCAAATGCAGAGG
TTAAGCAAGTCGTTTATAATGATACACTTTACAATGTATACATATCTGGTAATGACATGAAGCTTGAACCTGTCGTCTCG
CTTTCGGACATTAAAGTGGATGAGAATGGATATGTGAAAATTTTAGAGACGGCTGTAGAACTTACCGGCGTGTATGATCT
ATTTAAAGCAGCCACGGGCAGAGATCCGGTATCAGGCGAAAAAGTCACAGGGAAAGACCGTGTTGTTGCCTCCATAAACT
CAGTACCATTTGCCAAAATAGCTAAGTTAGAAAAGTTAATAGATATTAATAAACTTATTAATAATGGGAAAAAGGCTAAG
AAGGCTTCGGAAGTTAAAAACGTTGCTAAGGATAAAGGGAAAATTGCGAATGATGTAAGTGGATCTGCTAATAAAATTAA
TTCTGATTTAATAAAAAAGTATGCTAGAGATATAGAACAACGCACAGGTAGAGAATTACCTAAAAATCAAATAGATAAAT
TAAAGGAAGCATTAAGAAATAAAGAATATAAAAAAATGTCACCAATAGAGACTGCAAAACACAGGACTAAATTTGATAAA
GTAAAAAATAAAGTTATAAAAGAATGGGAAGAAAACACTGGACAAAAATGGCCTGTTTATAAGGAAAATGTTGTATCTGA
AAAAACAGGTAAAATTATTAGAAAAAAAGGGGATAAGTATGATGCTCACCATATAATTGAAAATACTTTTGGCGGAGAAC
ATGAATGGTGGAATATGCATCCAGCAAAATTTCCTAACGAGCATCAAGCTGGGATTCATGGTACAGGCTCACCAGCAAAT
GAATTATTTAAAGGAGGCAAAAAAAAATGA
TTGAAAACATTAGATGTCCACGCTCTTCACGAGGGCATTCAGCATACGATTGAGAAACTGGACAAGCAAAAACAACAGCT
GGAGAAGCTGGAAAAAAGCGTTGAGCATCTGGCAGGCATGAAGGATGCGCTGAAAGGAAAAGGCGGAGATGCCATCCGGA
CCTTTTACGAGGAGTGCCACAAGCCTTTTCTCCTTTTCTTTGGCATATTTATCGACGAATACAAAAAAGTGCTGAAACAA
ACACAGCATGCGATTTCCTCTGTAGAATCTAACTCCCACGGCATGATCGCAGAGGCTTTTCTCTCACACGATGCGAGACA
TGGGGTCAAGCATGCACGAGAAGTGACAGAGCAGCTCACTGACGCAGTCAATCGCCAGACATCAGCCATAGATCACATCG
TCAGCCTGCCGACAGTCAATGATTCCTTTTTTCGGATGGAAACAGAACAGGCTGAGCGGCTGATTTCAGACACCCTGAAT
AAGCTCTTTCAATTTGACGGCCAGCAAACCCAAGCACTAGAAGCCGCAAAATCAGATTTTCAAACGATGAAAAAATACAT
TGATCAGCTCGAAACCATGTACACCGGCCCGAAAATTGAAATCACAGGCTACAAAAGCGGCTCCATCCTGAAATCACAGG
AAGAAGAAAACATCAACCAAATCTTCGGCGCGATTAACCCGCAAATGAAGCAGGCGGATGATTCACCGATGGAGATGATG
CTGAAGAAATTAGCCGAGAATGAAAAGTCTAAAGTGGATTCAGTTGTCAAAACGGGTGATTCTAAAAAGGTAAGTAAAAA
TATCATCGTTATCAATGGTAAAGTGTATAATACGAGTGAACATAGAGAACATATAAAAACTGATTTCTCAAATGCAGAGG
TTAAGCAAGTCGTTTATAATGATACACTTTACAATGTATACATATCTGGTAATGACATGAAGCTTGAACCTGTCGTCTCG
CTTTCGGACATTAAAGTGGATGAGAATGGATATGTGAAAATTTTAGAGACGGCTGTAGAACTTACCGGCGTGTATGATCT
ATTTAAAGCAGCCACGGGCAGAGATCCGGTATCAGGCGAAAAAGTCACAGGGAAAGACCGTGTTGTTGCCTCCATAAACT
CAGTACCATTTGCCAAAATAGCTAAGTTAGAAAAGTTAATAGATATTAATAAACTTATTAATAATGGGAAAAAGGCTAAG
AAGGCTTCGGAAGTTAAAAACGTTGCTAAGGATAAAGGGAAAATTGCGAATGATGTAAGTGGATCTGCTAATAAAATTAA
TTCTGATTTAATAAAAAAGTATGCTAGAGATATAGAACAACGCACAGGTAGAGAATTACCTAAAAATCAAATAGATAAAT
TAAAGGAAGCATTAAGAAATAAAGAATATAAAAAAATGTCACCAATAGAGACTGCAAAACACAGGACTAAATTTGATAAA
GTAAAAAATAAAGTTATAAAAGAATGGGAAGAAAACACTGGACAAAAATGGCCTGTTTATAAGGAAAATGTTGTATCTGA
AAAAACAGGTAAAATTATTAGAAAAAAAGGGGATAAGTATGATGCTCACCATATAATTGAAAATACTTTTGGCGGAGAAC
ATGAATGGTGGAATATGCATCCAGCAAAATTTCCTAACGAGCATCAAGCTGGGATTCATGGTACAGGCTCACCAGCAAAT
GAATTATTTAAAGGAGGCAAAAAAAAATGA
Antitoxin
Download Length: 148 a.a. Molecular weight: 17664.53 Da Isoelectric Point: 3.8717
>AT6030 NP_391808.1 NC_000964:c4036787-4036344 [Bacillus subtilis subsp. subtilis str. 168]
MSYNFIKSNKENDFYPVNQSEIEEVEKNLNLKLPSELVNFYLEVGYGFIKGSEFNTNRILDPYSVRDFRLRINDFEFYPD
IEIYDEFENDKLIFFEGSESALMSIELNDNNKNPIYYYDIQIATSLTEFLRKIEENDQYYLDLLDDE
MSYNFIKSNKENDFYPVNQSEIEEVEKNLNLKLPSELVNFYLEVGYGFIKGSEFNTNRILDPYSVRDFRLRINDFEFYPD
IEIYDEFENDKLIFFEGSESALMSIELNDNNKNPIYYYDIQIATSLTEFLRKIEENDQYYLDLLDDE
Download Length: 444 bp
>AT6030 NC_000964:c4036787-4036344 [Bacillus subtilis subsp. subtilis str. 168]
ATGAGTTATAATTTTATAAAATCAAACAAAGAAAATGATTTTTATCCAGTGAATCAAAGTGAGATTGAAGAGGTTGAAAA
AAATTTAAACTTAAAATTACCTAGTGAATTAGTGAATTTTTATTTAGAAGTTGGCTATGGGTTTATTAAAGGATCAGAGT
TTAATACTAATCGCATTTTGGATCCATATTCTGTAAGAGACTTTCGATTGAGGATAAATGACTTTGAATTTTATCCTGAC
ATAGAAATATATGATGAGTTTGAGAATGATAAATTAATATTTTTTGAAGGTAGTGAATCGGCATTAATGTCAATTGAATT
AAATGATAATAATAAAAATCCGATTTACTATTATGATATTCAAATTGCAACTTCTCTTACCGAGTTCTTAAGAAAAATAG
AAGAGAATGATCAGTATTATCTTGATTTATTAGATGATGAATGA
ATGAGTTATAATTTTATAAAATCAAACAAAGAAAATGATTTTTATCCAGTGAATCAAAGTGAGATTGAAGAGGTTGAAAA
AAATTTAAACTTAAAATTACCTAGTGAATTAGTGAATTTTTATTTAGAAGTTGGCTATGGGTTTATTAAAGGATCAGAGT
TTAATACTAATCGCATTTTGGATCCATATTCTGTAAGAGACTTTCGATTGAGGATAAATGACTTTGAATTTTATCCTGAC
ATAGAAATATATGATGAGTTTGAGAATGATAAATTAATATTTTTTGAAGGTAGTGAATCGGCATTAATGTCAATTGAATT
AAATGATAATAATAAAAATCCGATTTACTATTATGATATTCAAATTGCAACTTCTCTTACCGAGTTCTTAAGAAAAATAG
AAGAGAATGATCAGTATTATCTTGATTTATTAGATGATGAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10057 | Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10 strain NCIB 3610 |
100 |
100 |
1 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10057 | Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10 strain NCIB 3610 |
100 |
100 |
1 |
Multiple sequence alignment
References
(1) Laura E Holberger et al. (2012) A novel family of toxin/antitoxin proteins in Bacillus species. FEBS Letters 586(2):132-6. [PubMed:22200572]
(2) Kazuo Kobayashi (2021) Diverse LXG toxin and antitoxin systems specifically mediate intraspecies competition in Bacillus subtilis biofilms. PLoS Genetics 17(7):e1009682. [PubMed:34280190]