Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1350267..1350898 Replicon chromosome
Accession NZ_CP012735
Organism Shigella flexneri 1a strain 0228

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag AOT98_RS07710 Protein ID WP_001259384.1
Coordinates 1350267..1350542 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AOT98_RS07715 Protein ID WP_000593555.1
Coordinates 1350539..1350898 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOT98_RS07695 (1345285) 1345285..1346352 + 1068 WP_000361470.1 HlyD family secretion protein -
AOT98_RS07700 (1346349) 1346349..1349084 + 2736 WP_000149170.1 ribosome-associated ATPase/putative transporter RbbA -
AOT98_RS07705 (1349084) 1349084..1350208 + 1125 WP_001216257.1 ABC transporter permease -
AOT98_RS07710 (1350267) 1350267..1350542 + 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
AOT98_RS07715 (1350539) 1350539..1350898 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AOT98_RS07720 (1351018) 1351018..1351419 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
AOT98_RS07725 (1351425) 1351425..1352231 - 807 WP_000173660.1 nickel import ATP-binding protein NikE -
AOT98_RS07730 (1352228) 1352228..1352992 - 765 WP_001136253.1 nickel import ATP-binding protein NikD -
AOT98_RS07735 (1352992) 1352992..1353825 - 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
AOT98_RS07740 (1353822) 1353822..1354766 - 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1329880..1350898 21018


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T57087 WP_001259384.1 NZ_CP012735:1350267-1350542 [Shigella flexneri 1a]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T57087 NZ_CP012735:1350267-1350542 [Shigella flexneri 1a]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT57087 WP_000593555.1 NZ_CP012735:1350539-1350898 [Shigella flexneri 1a]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT57087 NZ_CP012735:1350539-1350898 [Shigella flexneri 1a]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References