Detailed information of TA system TA056293

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2247445..2247641
• Replicon: chromosome
• Accession: NZ_CP012325
• Organism: Clostridioides difficile strain DSM 29020

Toxin (Protein)

• Gene name: CD1418.2
• Uniprot ID: D5Q3X5
• Locus tag: CDIF29020_RS11060
• Protein ID: WP_003424190.1
• Coordinates: 2247489..2247641 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	CD630_n00500
Locus tag	-
Coordinates	2247445..2247545 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF29020_RS11045	2242647..2242904	-	258	WP_003424182.1	hypothetical protein	-
CDIF29020_RS11050	2242891..2243124	-	234	WP_003424184.1	hypothetical protein	-
CDIF29020_RS11055	2243312..2243500	+	189	WP_003424185.1	helix-turn-helix transcriptional regulator	-
-	2247445..2247545	+	101	NuclAT_0	-	Antitoxin
CDIF29020_RS11060	2247489..2247641	-	153	WP_003424190.1	hypothetical protein	Toxin
CDIF29020_RS11070	2248469..2249014	+	546	WP_077699404.1	site-specific integrase	-
CDIF29020_RS11075	2249559..2250404	+	846	WP_003424194.1	DUF4878 domain-containing protein	-
CDIF29020_RS11080	2250628..2250792	-	165	WP_003424195.1	hypothetical protein	-
CDIF29020_RS11085	2251324..2251655	-	332	Protein_2017	carboxymuconolactone decarboxylase family protein	-
CDIF29020_RS11090	2251915..2252556	-	642	WP_003424198.1	SdpI family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	ant(6)-Ia / tet(40)	-	2023382..2329461	306079

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type I toxin-antitoxin system, toxin Ldr (1-35)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5744.78 Da
• Isoelectric Point: 10.6276

>T56293 WP_003424190.1 NZ_CP012325:c2247641-2247489 [Clostridioides difficile]
MDNFLQGILASLVASLIVYLTSKLFKKVKSHSGRSDFSFELEIKFKKNKH

Nucleotide

• Length: 153 bp

>T56293 NZ_CP012325:c2247641-2247489 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGTATATTAGCAAGTTTAGTTGCCAGTTTAATAGTTTACTTAACTAGTAAGTTATTTAAAAA
AGTAAAAAGCCACTCTGGCAGGAGTGACTTTAGTTTTGAACTAGAAATCAAGTTCAAAAAGAATAAACATTAG

Antitoxin

Nucleotide

• Length: 101 bp

>AT56293 NZ_CP012325:2247445-2247545 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTAGACGTAGAGTGAAGTTCAAATACTAATGTTTATTCTTTTTGAACTTGATTTCTAGTTC
AAAACTAAAGTCACTCCTGCC

Structures

Toxin

• AlphaFold DB: A0A449XCJ1

Antitoxin