Detailed information of TA system TA056286

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1709575..1709789
• Replicon: chromosome
• Accession: NZ_CP012323
• Organism: Clostridioides difficile strain DSM 28669

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: CDIF28669_RS08285
• Protein ID: WP_003429855.1
• Coordinates: 1709575..1709736 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1709656..1709789 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF28669_RS08235	1704915..1705148	+	234	WP_070113070.1	hypothetical protein	-
CDIF28669_RS08240	1705158..1705559	+	402	WP_077726212.1	hypothetical protein	-
CDIF28669_RS08245	1705553..1705900	+	348	WP_070113072.1	hypothetical protein	-
CDIF28669_RS08250	1705900..1706325	+	426	WP_038812299.1	HK97 gp10 family phage protein	-
CDIF28669_RS08255	1706318..1706755	+	438	WP_003433544.1	hypothetical protein	-
CDIF28669_RS08260	1706748..1706924	+	177	WP_070112848.1	hypothetical protein	-
CDIF28669_RS08265	1706925..1708235	+	1311	WP_070112850.1	phage tail sheath family protein	-
CDIF28669_RS08270	1708252..1708722	+	471	WP_003429851.1	phage tail tube protein	-
CDIF28669_RS08275	1708794..1709234	+	441	WP_003429853.1	phage portal protein	-
CDIF28669_RS08285	1709575..1709736	+	162	WP_003429855.1	hypothetical protein	Toxin
-	1709656..1709789	-	134	NuclAT_0	-	Antitoxin
-	1709656..1709789	-	134	NuclAT_1	-	Antitoxin
-	1710134..1710182	-	49	NuclAT_5	-	-
-	1710134..1710182	-	49	NuclAT_7	-	-
-	1711096..1711201	-	106	NuclAT_4	-	-
-	1711096..1711201	-	106	NuclAT_6	-	-
CDIF28669_RS08290	1712218..1712388	+	171	WP_021361718.1	ribbon-helix-helix domain-containing protein	-
CDIF28669_RS08295	1712516..1713358	+	843	WP_074048061.1	hypothetical protein	-
CDIF28669_RS08300	1713411..1713569	+	159	WP_021401161.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1678945..1754463	75518

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T56286 WP_003429855.1 NZ_CP012323:1709575-1709736 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T56286 NZ_CP012323:1709575-1709736 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCATAAGTTCAAAT
AG

Antitoxin

Nucleotide

• Length: 134 bp

>AT56286 NZ_CP012323:c1709789-1709656 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin