Detailed information of TA system TA056280

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2530784..2530986
• Replicon: chromosome
• Accession: NZ_CP012321
• Organism: Clostridioides difficile strain DSM 28666

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: CDIF28666_RS12145
• Protein ID: WP_004454589.1
• Coordinates: 2530834..2530986 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	2530784..2530913 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF28666_RS12110	2525896..2526213	+	318	WP_009897465.1	hypothetical protein	-
CDIF28666_RS12115	2526349..2526813	+	465	WP_004454576.1	hypothetical protein	-
CDIF28666_RS12125	2527142..2527303	-	162	WP_004454578.1	hypothetical protein	-
CDIF28666_RS12130	2527355..2527807	-	453	WP_021359435.1	hypothetical protein	-
CDIF28666_RS12135	2527804..2528169	-	366	WP_016729316.1	Bro-N domain-containing protein	-
CDIF28666_RS19230	2528289..2528441	-	153	WP_009897477.1	hypothetical protein	-
CDIF28666_RS12140	2529756..2530640	-	885	WP_021371352.1	hypothetical protein	-
-	2530784..2530913	+	130	NuclAT_1	-	Antitoxin
CDIF28666_RS12145	2530834..2530986	-	153	WP_004454589.1	hypothetical protein	Toxin
CDIF28666_RS12150	2531260..2531583	-	324	WP_009897482.1	hypothetical protein	-
CDIF28666_RS12155	2531619..2531873	-	255	WP_004454592.1	hypothetical protein	-
CDIF28666_RS12160	2532181..2532678	-	498	Protein_2250	transposase	-
CDIF28666_RS12165	2532968..2533343	-	376	Protein_2251	BlaI/MecI/CopY family transcriptional regulator	-
CDIF28666_RS12170	2533448..2533825	-	378	WP_021371356.1	BlaI/MecI/CopY family transcriptional regulator	-
CDIF28666_RS12175	2534630..2535124	+	495	WP_011861519.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
CDIF28666_RS12180	2535513..2535683	+	171	WP_021394397.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T56280 WP_004454589.1 NZ_CP012321:c2530986-2530834 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T56280 NZ_CP012321:c2530986-2530834 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT56280 NZ_CP012321:2530784-2530913 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin