Detailed information of TA system TA056253

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1725632..1725846
• Replicon: chromosome
• Accession: NZ_CP012309
• Organism: Clostridioides difficile strain DSM 28668

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: CDIF28668_RS08305
• Protein ID: WP_003429855.1
• Coordinates: 1725632..1725793 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1725713..1725846 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF28668_RS08255	1720971..1721204	+	234	WP_009896624.1	hypothetical protein	-
CDIF28668_RS08260	1721214..1721615	+	402	WP_009888836.1	hypothetical protein	-
CDIF28668_RS08265	1721609..1721956	+	348	WP_003429844.1	hypothetical protein	-
CDIF28668_RS08270	1721956..1722381	+	426	WP_109420488.1	HK97 gp10 family phage protein	-
CDIF28668_RS08275	1722374..1722811	+	438	WP_118822224.1	hypothetical protein	-
CDIF28668_RS08280	1722804..1722980	+	177	WP_021362376.1	hypothetical protein	-
CDIF28668_RS08285	1722982..1724292	+	1311	WP_118822225.1	phage tail sheath family protein	-
CDIF28668_RS08290	1724309..1724779	+	471	WP_003429851.1	phage tail tube protein	-
CDIF28668_RS08295	1724851..1725291	+	441	WP_003429853.1	phage portal protein	-
CDIF28668_RS08305	1725632..1725793	+	162	WP_003429855.1	hypothetical protein	Toxin
-	1725713..1725846	-	134	NuclAT_1	-	Antitoxin
-	1725713..1725846	-	134	NuclAT_2	-	Antitoxin
-	1726191..1726296	-	106	NuclAT_4	-	-
-	1726191..1726296	-	106	NuclAT_5	-	-
CDIF28668_RS08310	1726506..1726688	+	183	WP_118822226.1	hypothetical protein	-
CDIF28668_RS08315	1726818..1727699	+	882	WP_009901511.1	hypothetical protein	-
CDIF28668_RS08320	1727771..1730113	+	2343	WP_118822628.1	tape measure protein	-
CDIF28668_RS08325	1730125..1730814	+	690	WP_118822227.1	LysM peptidoglycan-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1696319..1757522	61203

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T56253 WP_003429855.1 NZ_CP012309:1725632-1725793 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T56253 NZ_CP012309:1725632-1725793 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCATAAGTTCAAAT
AG

Antitoxin

Nucleotide

• Length: 134 bp

>AT56253 NZ_CP012309:c1725846-1725713 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin